Using the single-cell imaging system and orthotropic footpad injection to establish mouse models for experimental and spontaneous melanoma metastasis

Yanlin Yu; Arnulfo Mendoza; Glenn Merlino

doi:10.1016/j.xpro.2023.102349

Using the single-cell imaging system and orthotropic footpad injection to establish mouse models for experimental and spontaneous melanoma metastasis

STAR Protoc. 2023 Sep 15;4(3):102349. doi: 10.1016/j.xpro.2023.102349. Epub 2023 Jun 13.

Authors

Yanlin Yu¹, Arnulfo Mendoza², Glenn Merlino³

Affiliations

¹ Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: yuy@mail.nih.gov.
² Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
³ Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

Metastasis, a complex process, is responsible for most deaths in patients with cancer. Clinically relevant research models are indispensable to advancing our understanding of metastatic mechanisms and developing new treatments. We here describe detailed protocols to establish mouse models for melanoma metastasis using the single-cell imaging system and orthotropic footpad injection. The single-cell imaging system permits the tracking and quantification of early metastatic cell survival, while the orthotropic footpad transplantation mimics aspects of the complex metastatic process. For complete details on the use and execution of this protocol, please refer to Yu et al.¹^,².

Keywords: Cancer; Model Organisms.

Published by Elsevier Inc.

MeSH terms

Animals
Disease Models, Animal
Humans
Melanoma* / diagnostic imaging
Melanoma* / pathology
Mice
Neoplasm Transplantation