Fine mapping of a recessive leaf rust resistance locus on chromosome 2BS in wheat accession CH1539

Dece Sheng; Linyi Qiao; Xiaojun Zhang; Xin Li; Lifang Chang; Huijuan Guo; Shuwei Zhang; Fang Chen; Zhijian Chang

doi:10.1007/s11032-022-01318-4

Fine mapping of a recessive leaf rust resistance locus on chromosome 2BS in wheat accession CH1539

Mol Breed. 2022 Aug 29;42(9):52. doi: 10.1007/s11032-022-01318-4. eCollection 2022 Sep.

Authors

Dece Sheng^#¹, Linyi Qiao^#², Xiaojun Zhang², Xin Li², Lifang Chang², Huijuan Guo², Shuwei Zhang², Fang Chen², Zhijian Chang²

Affiliations

¹ College of Life Science, Shanxi University, Taiyuan, 030006 Shanxi China.
² College of Agronomy, Shanxi Province Key Laboratory of Crop Genetics and Gene Improvement, Shanxi Agricultural University, Taiyuan, 030031 Shanxi China.

^# Contributed equally.

Abstract

Leaf rust (LR), caused by Puccinia triticina (Pt), is one of the most important fungal diseases of wheat worldwide. The wheat accession CH1539 showed a high level of resistance to leaf rust. A mapping population of 184 recombinant inbred lines (RILs) was developed from a cross between the resistant accession CH1539 and the susceptible cultivar SY95-71. The RILs showed segregating infection responses to Puccinia triticina Eriks. (Pt) race THK at the seedling stage. Genetic analysis showed that leaf rust resistance was controlled by a monogenic gene, and the potential locus was temporarily named LrCH1539. Bulked segregant analysis (BSA) using a 35 K DArTseq array located LrCH1539 on the short arm of chromosome 2B. Subsequently, a genetic linkage map of LrCH1539 was constructed using the developed 2BS chromosome-specific markers, and its flanking markers were sxau-2BS136 and sxau-2BS81. An F₂ subpopulation with 3619 lines was constructed by crossing the resistant and susceptible lines selected from the RIL population. The inoculation identification results showed that LrCH1539 was recessively inherited and was fine-mapped to a 779.4-kb region between markers sxau-2BS47 and sxau-2BS255 at the end of 2BS. The linkage marker analysis showed that the positions of LrCH1539 and Lr16 were the same, but the identification results of the resistance spectrum indicated that the causal genes of the two might be different. The resistant materials reported in this study and the cosegregation marker can be used for marker-assisted selection breeding of leaf rust-resistant wheat cultivars.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-022-01318-4.

Keywords: Fine-mapping; Leaf rust; Seedling resistance; Wheat.

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