Transfer of the high-temperature adult-plant stripe rust resistance gene Yr62 in four Chinese wheat cultivars

Jianian Zhou; Xiaochen Zheng; Xiao Zhong; Wenjing Tan; Chunhua Ma; Yuqi Wang; Ran Tian; Suizhuang Yang; Xin Li; Chongjing Xia; Zhensheng Kang; Xianming Chen; Xinli Zhou

doi:10.1007/s11032-023-01393-1

Transfer of the high-temperature adult-plant stripe rust resistance gene Yr62 in four Chinese wheat cultivars

Mol Breed. 2023 May 22;43(6):44. doi: 10.1007/s11032-023-01393-1. eCollection 2023 Jun.

Authors

Affiliations

¹ Wheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan China.
² State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi China.
³ US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics, and Quality Research Unit and Department of Plant Pathology, Washington State University, Pullman, WA USA.

PMID: 37313219
PMCID: PMC10248641 (available on 2024-05-22)
DOI: 10.1007/s11032-023-01393-1

Abstract

Wheat stripe rust is one of the diseases that seriously affect wheat production worldwide. Breeding resistant cultivars is an effective way to control this disease. The wheat stripe rust resistance gene Yr62 has high-temperature adult-plant resistance (HTAP). In this study, PI 660,060, a single Yr62 gene line, was crossed with four Chinese wheat cultivars, LunXuan987 (LX987), Bainongaikang58 (AK58), ZhengMai9023 (ZM9023), and HanMai6172 (H6172). F₁ seeds of four cross combinations were planted and self-crossed to develop the advance generations in the field. The seeds of each cross were mixed harvested and about 2400 to 3000 seeds were sown in each generation for F₁ to F₄ to maintain the maximum possible genotypes. Forty-five lines were selected and evaluated for resistance to stripe rust and agronomic traits, including plant height, number of grains per spike, and tiller number, in F₅ and F₆. Then, 33 lines with good agronomic traits and high disease resistance were developed to F₉ generation. SSR markers Xgwm251 and Xgwm192 flank linked with the Yr62 were used to detect the presence of Yr62 in these 33 F₉ lines. Of these, 22 lines were confirmed with the resistance gene Yr62. Finally, nine lines with good agronomic traits and disease resistance were successfully selected. The selected wheat lines in this study provide material support for the future breeding of wheat for stripe rust resistance.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-023-01393-1.

Keywords: Disease resistance breeding; HTAP resistance; SSR maker detection; Stripe rust; Wheat; Yr62.

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