Efficient identification of palatability-related genes using QTL mapping in rice breeding

Yoon-Hee Jang; Jae-Ryoung Park; Eun-Gyeong Kim; Rahmatullah Jan; Saleem Asif; Muhammad Farooq; Dan-Dan Zhao; Kyung-Min Kim

doi:10.1007/s11032-023-01392-2

Efficient identification of palatability-related genes using QTL mapping in rice breeding

Mol Breed. 2023 May 5;43(5):39. doi: 10.1007/s11032-023-01392-2. eCollection 2023 May.

Authors

Yoon-Hee Jang¹, Jae-Ryoung Park^{1

2}, Eun-Gyeong Kim¹, Rahmatullah Jan¹, Saleem Asif³, Muhammad Farooq³, Dan-Dan Zhao⁴, Kyung-Min Kim^{1

3}

Affiliations

¹ Coastal Agriculture Research Institute, Kyungpook National University, Daegu, 41566 Korea.
² Crop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju, 55365 Korea.
³ Department of Applied Biosciences, Kyungpook National University, Daegu, 41566 Korea.
⁴ Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration, Wanju, 55365 Korea.

PMID: 37312747
PMCID: PMC10248614 (available on 2024-05-05)
DOI: 10.1007/s11032-023-01392-2

Abstract

The gelatinization temperature of rice is an important factor in determining the eating and cooking quality, and it affects consumer preference. The alkali digestion value (ADV) is one of the main methods used to test the quality of rice and has a high correlation with the gelatinization temperature. For the development of high-quality rice, it is important to understand the genetic basis of palatability-related traits, and QTL analysis is a statistical method linking phenotypic data and genotype data and is an effective method to explain the genetic basis of variation in complex traits. QTL mapping related to the ADV of brown and milled rice was performed using the 120 Cheongcheong/Nagdong double haploid (CNDH) line. As a result, 12 QTLs related to ADV were detected, and 20 candidate genes were selected from the RM588-RM1163 region of chromosome 6 through screening by gene function analysis. The comparison of the relative expression level of candidate genes showed that OsSS1q6 is highly expressed in CNDH lines with high ADV in both brown rice and milled rice. In addition, OsSS1q6 has high homology with the starch synthase 1 protein and interacts with various starch biosynthesis-related proteins, such as GBSSII, SBE, and APL. Therefore, we suggest that OsSS1q6 identified through QTL mapping could be one of the various genes involved in the gelatinization temperature of rice by regulating starch biosynthesis. This study can be used as basic data for breeding high-quality rice and provides a new genetic resource that can increase the palatability of rice.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-023-01392-2.

Keywords: Alkali digestion value; Breeding; Eating and cooking quality; QTL; Rice.

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