Efficient Metabolic Fingerprinting of Follicular Fluid Encodes Ovarian Reserve and Fertility

Jiao Wu; Chunmei Liang; Xin Wang; Yida Huang; Wanshan Liu; Ruimin Wang; Jing Cao; Xun Su; Tao Yin; Xiaolei Wang; Zhikang Zhang; Lingchao Shen; Danyang Li; Weiwei Zou; Ji Wu; Lihua Qiu; Wen Di; Yunxia Cao; Dongmei Ji; Kun Qian

doi:10.1002/advs.202302023

Efficient Metabolic Fingerprinting of Follicular Fluid Encodes Ovarian Reserve and Fertility

Adv Sci (Weinh). 2023 Aug;10(23):e2302023. doi: 10.1002/advs.202302023. Epub 2023 Jun 13.

Authors

Jiao Wu^{1

2

3}, Chunmei Liang^{4

5

6}, Xin Wang⁴, Yida Huang^{1

2

3}, Wanshan Liu^{1

2

3}, Ruimin Wang^{1

2

3}, Jing Cao^{1

2

3}, Xun Su⁴, Tao Yin⁴, Xiaolei Wang⁴, Zhikang Zhang⁴, Lingchao Shen⁴, Danyang Li⁴, Weiwei Zou^{4

5

6}, Ji Wu^{7

8}, Lihua Qiu^{1

9}, Wen Di^{1

9}, Yunxia Cao^{4

5

6}, Dongmei Ji^{4

5

6}, Kun Qian^{1

2

3}

Affiliations

¹ Shanghai Key Laboratory of Gynecologic Oncology, Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P. R. China.
² State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering and Institute of Medical Robotics, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
³ Division of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P. R. China.
⁴ Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, P. R. China.
⁵ NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, Hefei, 230032, P. R. China.
⁶ Key Laboratory of Population Health Across Life Cycle, Anhui Medical University, Hefei, 230032, P. R. China.
⁷ Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Renji Hospital, Bio-X Institutes, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.
⁸ Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750003, P. R. China.
⁹ Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P. R. China.

Abstract

Ovarian reserve (OR) and fertility are critical in women's healthcare. Clinical methods for encoding OR and fertility rely on the combination of tests, which cannot serve as a multi-functional platform with limited information from specific biofluids. Herein, metabolic fingerprinting of follicular fluid (MFFF) from follicles is performed, using particle-assisted laser desorption/ionization mass spectrometry (PALDI-MS) to encode OR and fertility. PALDI-MS allows efficient MFFF, showing fast speed (≈30 s), high sensitivity (≈60 fmol), and desirable reproducibility (coefficients of variation <15%). Further, machine learning of MFFF is applied to diagnose diminished OR (area under the curve of 0.929) and identify high-quality oocytes/embryos (p < 0.05) by a single PALDI-MS test. Meanwhile, metabolic biomarkers from MFFF are identified, which also determine oocyte/embryo quality (p < 0.05) from the sampling follicles toward fertility prediction in clinics. This approach offers a powerful platform in women's healthcare, not limited to OR and fertility.

Keywords: biofluids; biomarkers; diagnostics; mass spectrometry; metabolism.

MeSH terms

Animals
Female
Fertility
Follicular Fluid* / chemistry
Follicular Fluid* / metabolism
Oocytes / metabolism
Ovarian Reserve*
Reproducibility of Results

Abstract

MeSH terms

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