In Vitro Morphogenesis and Differentiation of Human Intestinal Epithelium in a Gut-on-a-Chip

Woojung Shin; Hyun Jung Kim

doi:10.1007/978-1-0716-3076-1_15

In Vitro Morphogenesis and Differentiation of Human Intestinal Epithelium in a Gut-on-a-Chip

Methods Mol Biol. 2023:2650:197-206. doi: 10.1007/978-1-0716-3076-1_15.

Authors

Woojung Shin^{1

2}, Hyun Jung Kim³

Affiliations

¹ Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA. Woojung.Shin@wyss.harvard.edu.
² Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. Woojung.Shin@wyss.harvard.edu.
³ Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA. kimh19@ccf.org.

PMID: 37310633
DOI: 10.1007/978-1-0716-3076-1_15

Abstract

The establishment of a three-dimensional (3D) epithelial structure and cytodifferentiation in vitro is necessary to recapitulate in vivo-relevant structure and function of the human intestine. Here, we describe an experimental protocol to build an organomimetic gut-on-a-chip microdevice that allows inducing 3D morphogenesis of human intestinal epithelium using Caco-2 cells or intestinal organoid cells. Under physiological flow and physical motions, intestinal epithelium spontaneously recreates 3D epithelial morphology in a gut-on-a-chip that offers enhanced mucus production, epithelial barrier, and longitudinal host-microbe co-culture. This protocol may provide implementable strategies to advance traditional in vitro static cultures, human microbiome studies, and pharmacological testing.

Keywords: Gut-on-a-chip; Human intestinal epithelium; In vitro model; Morphogenesis; Organoid.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Caco-2 Cells
Cell Differentiation
Humans
Intestinal Mucosa*
Lab-On-A-Chip Devices*
Morphogenesis

Abstract

Publication types

MeSH terms

Grants and funding