Intestinal organoids are three-dimensional structures derived from tissue-resident adult stem cells. These organoids recapitulate key aspects of epithelial biology and can be used to study homeostatic turnover of the corresponding tissue. Organoids can be enriched for the various mature lineages which allows studies of the respective differentiation processes and of the diverse cellular functions. Here we describe mechanisms of intestinal fate specification and how these can be exploited to drive mouse and human small intestinal organoids into each of the functionally mature lineages.
Keywords: Adult stem cell-derived organoids; Differentiation; Intestinal lineages; Organoids; Small intestine.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.