Defining Anorectal Transition Zone Heterogeneity Using Single-Cell RNA Sequencing

Louciné Mitoyan; Charlyne Gard; Sébastien Nin; Béatrice Loriod; Géraldine Guasch

doi:10.1007/978-1-0716-3076-1_8

Defining Anorectal Transition Zone Heterogeneity Using Single-Cell RNA Sequencing

Methods Mol Biol. 2023:2650:89-103. doi: 10.1007/978-1-0716-3076-1_8.

Authors

Louciné Mitoyan^#¹, Charlyne Gard^#², Sébastien Nin², Béatrice Loriod², Géraldine Guasch³

Affiliations

¹ Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Epithelial Stem Cells and Cancer Team, Marseille, France.
² Aix-Marseille University, INSERM, TAGC, TGML, Marseille, France.
³ Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Epithelial Stem Cells and Cancer Team, Marseille, France. geraldine.guasch-grangeon@inserm.fr.

^# Contributed equally.

PMID: 37310626
DOI: 10.1007/978-1-0716-3076-1_8

Abstract

Special regions called transition zones (TZs) are found at numerous places in the body. TZs represent the junction between two different types of epithelia and are located between the esophagus and the stomach, in the cervix, in the eye, and between the anal canal and the rectum. TZ is a heterogeneous population, and the detailed characterization of its populations requires an analysis at the single-cell level. In this chapter, we provide a protocol to do single-cell RNA sequencing primary analysis of anal canal, TZ, and rectum epithelia.

Keywords: Anorectal transition zone; Cellular heterogeneity; Epithelial cells; Intestine; Rectum; Single-cell RNA sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Epithelium
Female
Humans
RNA*
Rectum*
Sequence Analysis, RNA
Stomach

Substances

RNA