Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens

Jung-Hoon Kwon; Kateri Bertran; Dong-Hun Lee; Miria Ferreira Criado; Lindsay Killmaster; Mary J Pantin-Jackwood; David E Swayne

doi:10.1080/22221751.2023.2218945

Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens

Emerg Microbes Infect. 2023 Dec;12(1):2218945. doi: 10.1080/22221751.2023.2218945.

Authors

Jung-Hoon Kwon^{1

2}, Kateri Bertran^{1

3

4}, Dong-Hun Lee⁵, Miria Ferreira Criado^{1

6}, Lindsay Killmaster¹, Mary J Pantin-Jackwood¹, David E Swayne¹

Affiliations

¹ Agricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA, USA.
² College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
³ Unitat mixta d'Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Bellaterra, Spain.
⁴ IRTA. Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Bellaterra, Spain.
⁵ College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
⁶ Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA.

Abstract

Clade 2.3.4.4 Eurasian lineage H5Nx highly pathogenic avian influenza virus (HPAIV) has become the globally dominant clade and caused global outbreaks since 2014. The clade 2.3.4.4 viruses have evolved into eight hemagglutinin subgroups (2.3.4.4a-h). In this study, we evaluated the infectivity, pathobiology, and transmissibility of seven clade 2.3.4.4 viruses (two 2.3.4.4a, two 2.3.4.4b, one 2.3.4.4c and two 2.3.4.4e) in chickens. The two clade 2.3.4.4e viruses caused 100% mortality and transmissibility in chickens. However, clade 2.3.4.4a and c viruses showed 80-90% mortality and 67% transmissibility. Clade 2.3.4.4b viruses showed 100% mortality, but no transmission to co-housed chickens was observed based on lack of seroconversion. All the infected chickens died showing systemic infection, irrespective of subgroup. The results highlight that all the clade 2.3.4.4 HPAIVs used in this study caused high mortality in infected chickens, but the transmissibility of the viruses in chickens was variable in contrast to that of previous Eurasian-lineage H5N1 HPAIVs. Changes in the pathogenicity and transmissibility of clade 2.3.4.4 HPAIVs warrant careful monitoring of the viruses to establish effective control strategies.

Keywords: Avian influenza virus; chicken; clade 2.3.4.4; highly pathogenic avian influenza virus; infectivity; pathogenicity; transmissibility.

MeSH terms

Animals
Chickens
Disease Outbreaks
Influenza A Virus, H5N1 Subtype*
Influenza A virus*
Sepsis*

Grants and funding

This study was supported by the U.S. Department of Agriculture, Agricultural Research Service Project 6040-32000-081-00D. Jung-Hoon Kwon is supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through Animal Disease Management Technology Advancement Support Program, funded by Ministry of Agriculture, Food and Rural Affairs (1220160-2).