Time-Resolved Small-Angle X-Ray Scattering of Protein Cage Assembly

Daisuke Sato; Takaaki Hikima; Masamichi Ikeguchi

doi:10.1007/978-1-0716-3222-2_12

Time-Resolved Small-Angle X-Ray Scattering of Protein Cage Assembly

Methods Mol Biol. 2023:2671:211-218. doi: 10.1007/978-1-0716-3222-2_12.

Authors

Daisuke Sato¹, Takaaki Hikima², Masamichi Ikeguchi^{3

4}

Affiliations

¹ Department of Bioinformatics, Soka University, Hachioji, Tokyo, Japan.
² RIKEN SPring-8 Center, Sayo, Hyogo, Japan.
³ Department of Bioinformatics, Soka University, Hachioji, Tokyo, Japan. ikeguchi@soka.ac.jp.
⁴ Department of Biosciences, Soka University, Hachioji, Tokyo, Japan. ikeguchi@soka.ac.jp.

PMID: 37308647
DOI: 10.1007/978-1-0716-3222-2_12

Abstract

Recent improvements in X-ray detectors and synchrotron light sources have made it possible to measure time-resolved small-angle X-ray scattering (TR-SAXS) at millisecond time resolution. As an example, in this chapter we describe the beamline setup, experimental scheme, and the points that should be noted in stopped-flow TR-SAXS experiments for investigating the ferritin assembly reaction.

Keywords: Exposure time; Ferritin; Radiation damage; SAXS; Stopped-flow.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ferritins*
Scattering, Small Angle
Synchrotrons*
X-Ray Diffraction
X-Rays

Substances

Ferritins