Reassembly Design of Ferritin Cages

Yu Liu; Guanghua Zhao

doi:10.1007/978-1-0716-3222-2_3

Reassembly Design of Ferritin Cages

Methods Mol Biol. 2023:2671:69-78. doi: 10.1007/978-1-0716-3222-2_3.

Authors

Yu Liu¹, Guanghua Zhao²

Affiliations

¹ College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, China.
² College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, China. gzhao@cau.edu.cn.

PMID: 37308638
DOI: 10.1007/978-1-0716-3222-2_3

Abstract

The ferritin family is distributed in nearly all organisms and protects them from iron-induced oxidative damage. Besides, its highly symmetrical structure and biochemical features make it an appealing material for biotechnological applications, such as building blocks for multidimensional assembly, templates for nano-reactors, and scaffolds for encapsulation and delivery of nutrients and drugs. Moreover, it is of great significance to construct ferritin variants with different properties, size, and shape to further broaden its application. In this chapter, we present a routine process of the ferritin redesign and the characterization method of the protein structure to provide a feasible scheme.

Keywords: Characterization method; Computational design; Ferritin; Geometry regulation; Protein cages; Self-assembly.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotechnology
Ferritins*
Iron*
Nutrients
Oxidative Stress

Substances

Ferritins
Iron