Strong inhibition of xanthine oxidase and elastase of Baccharis trimera (Less.) DC stem extract and analysis of biologically active constituents

Seung-Yub Song; Sung-Ho Lee; Min-Suk Bae; Dae-Hun Park; Seung-Sik Cho

doi:10.3389/fphar.2023.1160330

Strong inhibition of xanthine oxidase and elastase of Baccharis trimera (Less.) DC stem extract and analysis of biologically active constituents

Front Pharmacol. 2023 May 25:14:1160330. doi: 10.3389/fphar.2023.1160330. eCollection 2023.

Authors

Seung-Yub Song^{1

2}, Sung-Ho Lee^{1

2}, Min-Suk Bae³, Dae-Hun Park⁴, Seung-Sik Cho^{1

2}

Affiliations

¹ Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, Republic of Korea.
² Department of Biomedicine, Health, and Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan, Jeonnam, Republic of Korea.
³ Department of Environmental Engineering, Mokpo National University, Muan, Jeonnam, Republic of Korea.
⁴ College of Oriental Medicine, Dongshin University, Naju-si, Jeonnam, Republic of Korea.

Abstract

Introduction: In the present study, strong xanthine oxidase and elastase activities of Baccharis trimera (Less) DC stem (BT) were evaluated and active ingredients were identified to determine the possibility of using BT extract as an anti-hyperuricemia (gout) and cosmetic functional material. Methods: Hot water, 20, 40, 60, 80, and 100% ethanolic extracts of BT were prepared. The hot water extract had the highest extraction yield whereas the 100% ethanolic extract had the lowest yield. Results and discussion: Antioxidant effects were investigated based on DPPH radical scavenging activity, reducing power, and total phenolic contents. The 80% ethanolic extract showed the highest antioxidant activity. However, the 100% ethanol BT extract showed strong xanthine oxidase and elastase inhibitory activities. Functional substances were thought to be caffeic acid and luteolin. Minor active substances such as o-coumaric acid, palmitic acid, naringenin, protocatechoic acid, and linoleic acid were identified. Through this study, we firstly reported evidence that BT stem extract could be used as functional materials with anti-hyperuricemia and skin disease improving effects. BT stem extract could be used as an anti-hyperuricemia (gout) natural drug or cosmetic material. For further study, practical studies such as optimizing BT extraction and functional experiments for hyperuricemia (gout) and skin wrinkle improvement are considered necessary.

Keywords: Baccharis trimera; anti-wrikle; elastase; hyperuricemia; xanthine oxidase.

Grants and funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2022R1A5A8033794) and this work was supported by a grant from the National Institute of Envrionment Research (NIER), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIER-2021-03-03-007).