Genomic characterization and seed transmission of a novel unclassified partitivirus infecting Polygonatum kingianum Coll. et Hemsl

Fan Zhang; Xiaoxia Su; Shaozhi Zhang; Maosen Wang; Tiantian Wang; Xue Zheng; Kuo Wu; Kuanyu Zheng; Zhongkai Zhang

doi:10.1016/j.heliyon.2023.e16719

Genomic characterization and seed transmission of a novel unclassified partitivirus infecting Polygonatum kingianum Coll. et Hemsl

Heliyon. 2023 May 26;9(6):e16719. doi: 10.1016/j.heliyon.2023.e16719. eCollection 2023 Jun.

Authors

Fan Zhang^{1

2

3}, Xiaoxia Su^{1

2}, Shaozhi Zhang^{1

2}, Maosen Wang^{1

2

3}, Tiantian Wang^{1

2}, Xue Zheng^{1

2}, Kuo Wu^{1

2}, Kuanyu Zheng^{1

2}, Zhongkai Zhang^{1

2}

Affiliations

¹ Biotechnology and Germplasm Resources Research Institute, Yunnan Academy of Agricultural Sciences, 2238# Beijing Rd, Panlong District, Kunming 650205, Yunnan, PR China.
² Yunnan Provincial Key Lab of Agricultural Biotechnology, 2238# Beijing Rd, Panlong District, Kunming 650205, Yunnan, PR China.
³ School of Agriculture, Yunnan University, Kunming, 650091, Yunnan, PR China.

Abstract

This study identified a novel virus in the family Partitiviridae infecting Polygonatum kingianum Coll. et Hemsl, which is tentatively named polygonatum kingianum cryptic virus 1 (PKCV1). PKCV1 genome has two RNA segments: dsRNA1 (1926 bp) has an open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 581 amino acids (aa), and dsRNA2 (1721 bp) has an ORF encoding a capsid protein (CP) of 495 aa. The RdRp of PKCV1 shares 20.70-82.50% aa identity with known partitiviruses, and the CP of PKCV1 shares 10.70-70.80% aa identity with known partitiviruses. Moreover, PKCV1 phylogenetically clustered with unclassified members of the Partitiviridae family. Additionally, PKCV1 is common in P. kingianum planting regions and has a high infection rate in P. kingianum seeds.

Keywords: CP; Partitiviridae; Polygonatum kingianum; RdRp; Seed transmission.