A gold nanoflower based dual mode aptasensor for aflatoxin B1 detection using SERS and fluorescence effect simultaneously

Zhiyi Song; Xinyi Wang; Peifang Chen; Zhouping Wang; Xiaoyuan Ma

doi:10.1016/j.saa.2023.122963

A gold nanoflower based dual mode aptasensor for aflatoxin B₁ detection using SERS and fluorescence effect simultaneously

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Nov 15:301:122963. doi: 10.1016/j.saa.2023.122963. Epub 2023 Jun 7.

Authors

Zhiyi Song¹, Xinyi Wang¹, Peifang Chen¹, Zhouping Wang¹, Xiaoyuan Ma²

Affiliations

¹ State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Collaborative innovation center of food safety and quality control in Jiangsu Province, Jiangnan University, Wuxi 214122, China.
² State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Collaborative innovation center of food safety and quality control in Jiangsu Province, Jiangnan University, Wuxi 214122, China. Electronic address: maxy@jiangnan.edu.cn.

PMID: 37302200
DOI: 10.1016/j.saa.2023.122963

Abstract

Aflatoxin B₁ (AFB₁) is usually the major aflatoxin produced by toxigenic strains and has been identified the most potent natural carcinogen. Here, a SERS/fluorescence dual-mode nanosensor has been designed while gold nanoflowers (AuNFs) was used as substrate for the detection of AFB₁. AuNFs exhibited excellent SERS enhancement effect as well as the good fluorescence quenching effect which made the dual signal detection possible. First, the surface of AuNFs was modified with AFB₁ aptamer via Au-SH group. Then, the complementary sequence functionalized with Cy5 (the signal molecule) was attached to AuNFs based on the base complementary pairing principle. On this case, Cy5 was close to AuNFs, the SERS intensity was greatly enhanced and the fluorescence intensity was quenched. After incubation with AFB₁, the aptamer was preferentially combined to its target AFB₁. Thus, the complementary sequence detached from AuNFs which caused the SERS intensity of Cy5 decreased while its fluorescence effect recovered. Then, the quantitative detection was realized with two optical properties. The LOD was calculated to be 0.03 ng/mL. It was a convenient and fast detection method which expanded the application of nanomaterials based multi-signal simultaneous detection.

Keywords: AFB(1); Dual-mode sensor; Fluorescence; Gold nanoflowers; SERS.

MeSH terms

Aflatoxin B1 / analysis
Aptamers, Nucleotide* / chemistry
Gold / chemistry
Limit of Detection
Metal Nanoparticles* / chemistry

Substances

Aflatoxin B1
Gold
cyanine dye 5
Aptamers, Nucleotide