Irradiation-induced IFN-type-I pathway activation in prostate cancer cell lines

Erasmia T Xanthopoulou; Ioannis M Koukourakis; Christos Kakouratos; Christos Nanos; Christos Kalaitzis; Alexandra Giatromanolaki; Michael I Koukourakis

doi:10.1016/j.cyto.2023.156252

Irradiation-induced IFN-type-I pathway activation in prostate cancer cell lines

Cytokine. 2023 Sep:169:156252. doi: 10.1016/j.cyto.2023.156252. Epub 2023 Jun 8.

Authors

Erasmia T Xanthopoulou¹, Ioannis M Koukourakis², Christos Kakouratos¹, Christos Nanos¹, Christos Kalaitzis³, Alexandra Giatromanolaki⁴, Michael I Koukourakis⁵

Affiliations

¹ Department of Radiotherapy / Oncology, Democritus University of Thrace, Alexandroupolis 68100, Greece.
² Radiation Oncology Unit, Aretaieion University Hospital, Athens, Greece.
³ Department of Urology, Democritus University of Thrace, Alexandroupolis 68100, Greece.
⁴ Department of Pathology, Democritus University of Thrace, Alexandroupolis 68100, Greece.
⁵ Department of Radiotherapy / Oncology, Democritus University of Thrace, Alexandroupolis 68100, Greece. Electronic address: targ@her.forthnet.gr.

PMID: 37301190
DOI: 10.1016/j.cyto.2023.156252

Abstract

The Interferon (ΙFN) Type-I pathway has an important role in the activation of an anti-tumor immune response. We investigated the effects of two different dose fractionations of radiation (3 daily 8 Gy fractions vs. one fraction of 20 Gy) on the activation of the Type-I IFN-pathway in three hormone-dependent (22Rv1) and independent (DU145, PC3), prostate cancer (PC) cell lines. Regardless of the dose schedules, radiation-induced the expression of IFN-stimulated genes in all PC cell lines, with a strong up-regulation of the IFI6v2 and IFI44 genes. In addition, strong up-regulation of the MX1 and MX2 genes was noted in the PC3 cell line. This effect was independent of the expression of IFNβ, cGAS, or TREX1 levels. It is suggested that the RT-induced IFN type-I response could be exploited for the development of immuno-RT policies for localized and metastatic PC.

Keywords: IFN-stimulated genes; Prostate cancer; Radiotherapy; Type-I Interferon Pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cell Line, Tumor
Humans
Interferon Type I*
Male
Prostatic Neoplasms* / genetics
Prostatic Neoplasms* / pathology
Prostatic Neoplasms* / radiotherapy

Substances

Interferon Type I