Selenium-mediated Cr(VI) reduction and SeNPs synthesis accelerated Bacillus cereus SES to remediate Cr contamination

Min Nie; Miaomiao Cai; Chihhung Wu; Shiqian Li; Suhua Chen; Guangyu Shi; Xu Wang; Chengxiao Hu; Jiatao Xie; Yanni Tang; Huan Zhang; Xiaohu Zhao

doi:10.1016/j.jhazmat.2023.131713

Selenium-mediated Cr(VI) reduction and SeNPs synthesis accelerated Bacillus cereus SES to remediate Cr contamination

J Hazard Mater. 2023 Sep 5:457:131713. doi: 10.1016/j.jhazmat.2023.131713. Epub 2023 May 25.

Authors

Min Nie¹, Miaomiao Cai², Chihhung Wu³, Shiqian Li⁴, Suhua Chen⁵, Guangyu Shi⁶, Xu Wang⁷, Chengxiao Hu², Jiatao Xie², Yanni Tang², Huan Zhang², Xiaohu Zhao⁸

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China; Fujian Provincial Key Laboratory of Resources and Environment Monitoring & Sustainable Management and Utilization, Sanming University, Sanming 365004, China.
² State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
³ Fujian Provincial Key Laboratory of Resources and Environment Monitoring & Sustainable Management and Utilization, Sanming University, Sanming 365004, China.
⁴ Fujian Provincial Key Lab of Coastal Basin Environment, Fujian Polytechnic Normal University, Fuqing 350300, China.
⁵ National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang 330063, China.
⁶ College of Environment Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
⁷ Institute of Quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
⁸ State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xhzhao@mail.hzau.edu.cn.

PMID: 37301074
DOI: 10.1016/j.jhazmat.2023.131713

Abstract

Microbial biotransformation of Cr(VI) is a sustainable approach to reduce Cr(VI) toxicity and remediate Cr(VI) contamination. In this study, Bacillus cereus SES with the capability of reducing both Cr(VI) and Se(IV) was isolated, and the effect of Se supplementation on Cr(VI) reduction by Bacillus cereus SES was investigated. Se(IV) addition enabled 2.6-fold faster Cr(VI) reduction, while B. cereus SES reduced 96.96% Se(IV) and produced more selenium nanoparticles (SeNPs) in the presence of Cr(VI). Co-reduction products of B. cereus SES on Cr(VI) and Se(IV) were SeNPs adsorbed with Cr(III). The relevant mechanisms were further revealed by proteomics. Se(IV) supplementation mediated the synthesis of Cr(VI) reductants and stress-resistant substances, thus enhancing Cr(VI) resistance and promoting Cr(VI) reduction. Meanwhile, high Se(IV) reduction rate was associated with Cr(VI)-induced electron transport processes, and Cr(VI) mediated the up-regulation of flagellar assembly, protein export and ABC transporters pathways to synthesis and export more SeNPs. Furthermore, Se combined with B. cereus SES had the potential to reduce the toxicity of Cr(VI) via reducing the bioavailability of Cr and improving the bioavailability of Se in soil. Results suggested that Se could be an efficient strategy to enhance the remediation of B. cereus SES on Cr contamination.

Keywords: Bacillus cereus; Cr(VI) bioremediation; Proteomics; Selenite reduction; Selenium nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus cereus / metabolism
Nanoparticles*
Oxidation-Reduction
Selenium* / metabolism
Selenium* / pharmacology

Substances

Selenium
chromium hexavalent ion