A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally Mediated Fluorescence, and Live Microscopy

Sophie Brown; Elaina Atherton; David A Borton

doi:10.1007/978-1-0716-3287-1_12

A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally Mediated Fluorescence, and Live Microscopy

Methods Mol Biol. 2023:2683:153-167. doi: 10.1007/978-1-0716-3287-1_12.

Authors

Sophie Brown^{1

2}, Elaina Atherton³, David A Borton^{4

5

6

7

8}

Affiliations

¹ Center for Biomedical Engineering, Brown University, Providence, RI, USA.
² School of Engineering, Brown University, Providence, RI, USA.
³ Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, USA.
⁴ Center for Biomedical Engineering, Brown University, Providence, RI, USA. david_borton@brown.edu.
⁵ Department of Veteran Affairs, Providence Medical Center, Center for Neurorestoration and Neurotechnology, Providence, RI, USA. david_borton@brown.edu.
⁶ Department of Neurosurgery, Rhode Island Hospital, Providence, RI, USA. david_borton@brown.edu.
⁷ School of Engineering, Brown University, Providence, RI, USA. david_borton@brown.edu.
⁸ Carney Institute for Brain Science, Brown University, Providence, RI, USA. david_borton@brown.edu.

PMID: 37300773
DOI: 10.1007/978-1-0716-3287-1_12

Abstract

In vitro cell culture models can offer high-resolution and high-throughput experimentation of cellular behaviors. However, in vitro culture approaches often fail to fully recapitulate complex cell processes involving synergistic interactions between heterogeneous neural cell populations and the surrounding neural microenvironment. Here, we describe the formation of a three-dimensional primary cortical cell culture system compatible with live confocal microscopy.

Keywords: In vitro; Live imaging; Primary cell culture; Three-dimensional cell culture.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Animals, Genetically Modified
Cell Culture Techniques* / methods
Imaging, Three-Dimensional / methods
Microscopy, Confocal
Microscopy, Fluorescence / methods
Neurons*

Grants and funding

R21 AG077697/AG/NIA NIH HHS/United States