Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors

Shih-Wei Chen; Yu-Hui Wong

doi:10.1007/978-1-0716-3287-1_5

Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors

Methods Mol Biol. 2023:2683:53-68. doi: 10.1007/978-1-0716-3287-1_5.

Authors

Shih-Wei Chen¹, Yu-Hui Wong²

Affiliations

¹ Department of Life Sciences and Institute of Genome Sciences, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.
² Brain Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan. yuhui.wong@nycu.edu.tw.

PMID: 37300766
DOI: 10.1007/978-1-0716-3287-1_5

Abstract

The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological disorders, as well as the carrying out of drug screening and toxicity testing. Here, we provide a stepwise protocol for the simple, robust, and efficient differentiation of hiPSCs into microglia-like cells (iMGs) by overexpression of SPI1 and CEBPA. This protocol details hiPSC culture, lentivirus production, lentivirus delivery, and, finally, the differentiation and validation of the iMG cells.

Keywords: CEBPA; Induced pluripotent stem cells; Microglia; SPI1; Transcription factor-based differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
Cells, Cultured
Humans
Induced Pluripotent Stem Cells*
Microglia
Transcription Factors

Substances

Transcription Factors