Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells

Peiyan Ni; Lingyi Fan; Amy Zinski; Sangmi Chung

doi:10.1007/978-1-0716-3287-1_2

Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells

Methods Mol Biol. 2023:2683:13-20. doi: 10.1007/978-1-0716-3287-1_2.

Authors

Peiyan Ni¹, Lingyi Fan¹, Amy Zinski², Sangmi Chung³

Affiliations

¹ Psychiatric Laboratory and Mental Health Center, The State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, China.
² Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY, USA.
³ Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY, USA. schung8@nymc.edu.

PMID: 37300763
DOI: 10.1007/978-1-0716-3287-1_2

Abstract

Cortical interneurons (cINs), especially those that are derived from the medial ganglionic eminence (MGE) during early development, are associated with various neuropsychiatric disorders. Human pluripotent stem cell (hPSC)-derived cINs can provide unlimited cell sources for studying disease mechanisms and developing novel therapeutics. Here, we describe an optimized method to generate homogeneous cIN populations based on three-dimensional (3D) cIN sphere generation. This optimized differentiation system could sustain generated cINs relatively long term without compromising their survival or phenotypes.

Keywords: Cortical interneurons (cINs); Human pluripotent stem cells (hPSCs); Medial ganglionic eminence (MGE); cIN spheres.

Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells

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