Lateral Filter Array Microfluidic Devices for Detecting Circulating Tumor Cells

Kangfu Chen; Thomas J George; Z Hugh Fan

doi:10.1007/978-1-0716-3271-0_1

Lateral Filter Array Microfluidic Devices for Detecting Circulating Tumor Cells

Methods Mol Biol. 2023:2679:1-13. doi: 10.1007/978-1-0716-3271-0_1.

Authors

Kangfu Chen¹, Thomas J George², Z Hugh Fan^{3

4

5}

Affiliations

¹ Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada.
² Department of Medicine, University of Florida, Gainesville, FL, USA.
³ Interdisciplinary Microsystems Group (IMG), Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, USA. hfan@ufl.edu.
⁴ J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA. hfan@ufl.edu.
⁵ Department of Chemistry, University of Florida, Gainesville, FL, USA. hfan@ufl.edu.

PMID: 37300605
DOI: 10.1007/978-1-0716-3271-0_1

Abstract

Circulating tumor cells (CTCs) are an important liquid biopsy biomarker for next-generation cancer diagnosis and prognosis. However, their clinical usage is hindered by the rarity of CTCs in patient's peripheral blood. Microfluidics has shown unique advantages in CTC isolation and detection. We have developed lateral filter array microfluidic (LFAM) devices for highly efficient CTC isolation. In this chapter, we describe in detail the design and fabrication of the LFAM devices and their applications for CTC enumeration from clinical blood samples.

Keywords: Circulating tumor cells; Filtration; Immunoaffinity; Microfluidics; Pancreatic cancer.

MeSH terms

Cell Line, Tumor
Cell Separation
Humans
Lab-On-A-Chip Devices
Liquid Biopsy
Microfluidic Analytical Techniques*
Microfluidics
Neoplastic Cells, Circulating* / pathology
Neuromuscular Diseases*