Decellularized periosteum promotes guided bone regeneration via manipulation of macrophage polarization

Jiayang Li; Dongming He; Longwei Hu; Siyi Li; Chenping Zhang; Xuelai Yin; Zhen Zhang

doi:10.1002/biot.202300094

Decellularized periosteum promotes guided bone regeneration via manipulation of macrophage polarization

Biotechnol J. 2023 Oct;18(10):e2300094. doi: 10.1002/biot.202300094. Epub 2023 Jun 23.

Authors

Jiayang Li^{1

2}, Dongming He³, Longwei Hu¹, Siyi Li¹, Chenping Zhang¹, Xuelai Yin¹, Zhen Zhang¹

Affiliations

¹ Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai Center of Head and Neck Oncology Clinical and Translational Science, Shanghai, China.
² Department of Endodontics, Shanghai Stomatological Hospital, Fudan University; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China.
³ Department of Oral & Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai Center of Head and Neck Oncology Clinical and Translational Science, Shanghai, China.

PMID: 37300523
DOI: 10.1002/biot.202300094

Abstract

Periosteum has shown potential as an effective barrier membrane for guided bone regeneration (GBR). However, if recognized as a "foreign body," insertion of a barrier membrane in GBR treatment will inevitably alter the local immune microenvironment and subsequently influence bone regeneration. The aim of this investigation was to fabricate decellularized periosteum (DP) and investigate its immunomodulatory properties in GBR. DP was successfully fabricated from periosteum from the mini-pig cranium. In vitro experiments indicated that the DP scaffold modulated macrophage polarization toward a pro-regenerative M2 phenotype, which in turn facilitated migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. A rat GBR model with a cranial critical-size defect was established, and our in vivo experiment confirmed the beneficial effects of DP on the local immune microenvironment and bone regeneration. Collectively, the findings of this study indicate that the prepared DP possesses immunomodulatory properties and represents a promising barrier membrane for GBR procedures.

Keywords: barrier membrane; decellularized periosteum; guided bone regeneration (GBR); osteoimmunomodulation.

Abstract

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