In order to study the detoxification effect of microbial exopolysaccharides (EPS) on the heavy metal cadmium (Cd2+), this study took an EPS-producing Serratia fonticola CPSE11 (NZ_CP050171.1) isolated from Codonopsis pilosula root as the research object. The whole genome and EPS synthesis gene clusters of this strain were predicted and analyzed, the adsorption kinetics of EPS on Cd2+ were studied by using pseudo-first-order and second-order kinetic equations, the isothermal adsorption curves were simulated and analyzed by using the Langmuir isothermal adsorption equation, and the effects of Cd2+ and EPS on the growth of C. pilosula were explored by seed germination experiment and hydroponic experiment. The analysis revealed that this strain contained three gene clusters related to EPS synthesis, and the metabolic pathway for EPS synthesis was obtained on the basis of the whole genome analysis and microbial physiological metabolism. The molecular weight and monosaccharide composition of EPS were determined by HPLC analysis, which showed that EPS consisted of mannose, glucosamine, rhamnose, galactosamine, glucose, and galactose with a molar ratio of 1:1.74:4.57:3.96:14.04:10.28, with the molecular weight of 366,316.09 kDa. The adsorption process of EPS on Cd2+ was in accordance with the second-order kinetic model, and the results of seed germination experiments showed that EPS could promote seed germination and improve seed activity. In the hydroponic experiment, high concentration of Cd2+ (15 mg/L) caused toxic symptoms in C. pilosula, while the addition of EPS reduced the toxic effect of Cd2+ on C. pilosula, and the plant growth was significantly improved.
Keywords: Cd2+; Codonopsis pilosula; Exopolysaccharides; Hydroponic experiment; Serratia fonticola CPSE11.
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.