Development and validation of a real-time PCR test to detect Bartonella quintana in clinical samples

Jamie Choat; Brook Yockey; Sarah W Sheldon; Ryan Pappert; Jeannine Petersen; Elizabeth A Dietrich

doi:10.1016/j.diagmicrobio.2023.116000

Development and validation of a real-time PCR test to detect Bartonella quintana in clinical samples

Diagn Microbiol Infect Dis. 2023 Aug;106(4):116000. doi: 10.1016/j.diagmicrobio.2023.116000. Epub 2023 May 28.

Authors

Jamie Choat¹, Brook Yockey¹, Sarah W Sheldon¹, Ryan Pappert¹, Jeannine Petersen¹, Elizabeth A Dietrich²

Affiliations

¹ Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.
² Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA. Electronic address: wul2@cdc.gov.

PMID: 37295184
PMCID: PMC10860707 (available on 2024-08-01)
DOI: 10.1016/j.diagmicrobio.2023.116000

Abstract

This study reports on the validation of a real-time polymerase chain reaction test targeting the vomp region of Bartonella quintana. The assay displayed 100% sensitivity and specificity for the 52 bloods and 159 cultures tested. Molecular diagnosis of Bartonella quintana can aid clinical treatment during acute infection.

Keywords: Clinical diagnostics; Laboratory method; Real-time PCR.

Published by Elsevier Inc.

MeSH terms

Bartonella henselae*
Bartonella quintana* / genetics
Humans
Real-Time Polymerase Chain Reaction

Grants and funding

CC999999/ImCDC/Intramural CDC HHS/United States