Bioactive dipeptides enhance the tolerance of lager yeast to ethanol-oxidation cross-stress by regulating the multilevel defense system

Caiyun Wu; Jiayu Guo; Haoyu Jian; Li Liu; Hexin Zhang; Nana Yang; Huaide Xu; Hongjie Lei

doi:10.1016/j.fm.2023.104288

Bioactive dipeptides enhance the tolerance of lager yeast to ethanol-oxidation cross-stress by regulating the multilevel defense system

Food Microbiol. 2023 Sep:114:104288. doi: 10.1016/j.fm.2023.104288. Epub 2023 Apr 19.

Authors

Caiyun Wu¹, Jiayu Guo², Haoyu Jian³, Li Liu⁴, Hexin Zhang⁵, Nana Yang⁶, Huaide Xu⁷, Hongjie Lei⁸

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: wucaiyun@nwafu.edu.cn.
² College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: guo.jiayu@nwafu.edu.cn.
³ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: jianhaoyu@nwafu.edu.cn.
⁴ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: liuli101@nwafu.edu.cn.
⁵ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: zhanghexin1217@nwafu.edu.cn.
⁶ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: yangnana@nwafu.edu.cn.
⁷ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: Huaide_Xu@163.com.
⁸ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: leihongjie@nwafu.edu.cn.

PMID: 37290871
DOI: 10.1016/j.fm.2023.104288

Abstract

Although high gravity brewing technology has been widely used for beer industries due to its economic benefits, yeast cells are subjected to multiple environmental stresses throughout the fermentation process. Eleven bioactive dipeptides (LH, HH, AY, LY, IY, AH, PW, TY, HL, VY, FC) were selected to evaluate their effects on cell proliferation, cell membrane defense system, antioxidant defense system and intracellular protective agents of lager yeast against ethanol-oxidation cross-stress. Results showed that the multiple stresses tolerance and fermentation performance of lager yeast were enhanced by bioactive dipeptides. Cell membrane integrity was improved by bioactive dipeptides through altering the structure of macromolecular compounds of the cell membrane. Intracellular reactive oxygen species (ROS) accumulation was significantly decreased by bioactive dipeptides, especially for FC, decreasing by 33.1%, compared with the control. The decrease of ROS was closely related to the increase of mitochondrial membrane potential, intracellular antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and glycerol level. In addition, bioactive dipeptides could regulate the expression of key genes (GPD1, OLE1, SOD2, PEX11, CTT1, HSP12) to enhance the multilevel defense systems under ethanol-oxidation cross-stress. Therefore, bioactive dipeptides should be potentially efficient and feasible bioactive ingredients to improve the multiple stresses tolerance of lager yeast during high gravity fermentation.

Keywords: Bioactive dipeptides; Ethanol-oxidation cross-stress; High gravity fermentation; Lager yeast.

MeSH terms

Antioxidants / metabolism
Beer
Ethanol / metabolism
Fermentation
Membrane Proteins
Peroxins / metabolism
Reactive Oxygen Species / metabolism
Saccharomyces cerevisiae Proteins* / metabolism
Saccharomyces cerevisiae* / metabolism

Substances

Reactive Oxygen Species
Antioxidants
Ethanol
PEX11 protein, S cerevisiae
Peroxins
Membrane Proteins
Saccharomyces cerevisiae Proteins

Supplementary concepts

Saccharomyces pastorianus