The time course of acclimation of critical thermal maxima is modulated by the magnitude of temperature change and thermal daily fluctuations

Abstract

Plasticity in the critical thermal maximum (CT_max) helps ectotherms survive in variable thermal conditions. Yet, little is known about the environmental mechanisms modulating its time course. We used the larvae of three neotropical anurans (Boana platanera, Engystomops pustulosus and Rhinella horribilis) to test whether the magnitude of temperature changes and the existence of fluctuations in the thermal environment affected both the amount of change in CT_max and its acclimation rate (i.e., its time course). For that, we transferred tadpoles from a pre-treatment temperature (23 °C, constant) to two different water temperatures: mean (28 °C) and hot (33 °C), crossed with constant and daily fluctuating thermal regimes, and recorded CT_max values, daily during six days. We modeled changes in CT_max as an asymptotic function of time, temperature, and the daily thermal fluctuation. The fitted function provided the asymptotic CT_max value (CT_max∞) and CT_max acclimation rate (k). Tadpoles achieved their CT_max∞ between one and three days. Transferring tadpoles to the hot treatment generated higher CT_max∞ at earlier times, inducing faster acclimation rates in tadpoles. In contrast, thermal fluctuations equally led to higher CT_max∞ values but tadpoles required longer times to achieve CT_max∞ (i.e., slower acclimation rates). These thermal treatments interacted differently with the studied species. In general, the thermal generalist Rhinella horribilis showed the most plastic acclimation rates whereas the ephemeral-pond breeder Engystomops pustulosus, more exposed to heat peaks during larval development, showed less plastic (i.e., canalized) acclimation rates. Further comparative studies of the time course of CT_max acclimation should help to disentangle the complex interplay between the thermal environment and species ecology, to understand how tadpoles acclimate to heat stress.

Keywords: Acclimation rate; Acclimation temperature; Amphibians; CT(max); Daily thermal fluctuations; Phenotypic plasticity.