Study on skin infection model of Staphylococcus aureus based on analytic hierarchy process and Delphi method

Jiaxin Liang; Guofa Zou; Chiming Gu; Shuhong Tao; Libing Guo; Chunping Tang; Jinhong Zhang; Zujun Deng; Yanfen Chen

doi:10.1016/j.heliyon.2023.e16327

Study on skin infection model of Staphylococcus aureus based on analytic hierarchy process and Delphi method

Heliyon. 2023 May 16;9(6):e16327. doi: 10.1016/j.heliyon.2023.e16327. eCollection 2023 Jun.

Authors

Jiaxin Liang¹, Guofa Zou¹, Chiming Gu², Shuhong Tao¹, Libing Guo¹, Chunping Tang¹, Jinhong Zhang¹, Zujun Deng¹, Yanfen Chen^{1

3}

Affiliations

¹ Guangdong Pharmaceutical University, Guangzhou, PR China.
² The Second Clinical Medical College of Guangzhou University of Chinese Medicine; Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, PR China.
³ Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems, Guangzhou, PR China.

Abstract

Purpose: Infectious skin diseases are a type of inflammatory skin lesions caused by pathogenic microorganisms. Because of the uncertainty of methodology, the skin infection model usually have low replication rate and lack of good evaluation system. We aimed to establish multi-index and comprehensive evaluation method for Staphylococcus aureus (S.aureus) skin-infection models through Analytic hierarchy process (AHP) and Delphi method, and screen high quality animal models through it.

Materials and methods: Firstly, the evaluation indicators of skin infection were collected basing on literature research. The weight of the evaluation indicators were decided according to AHP and Delphi method. Then different ulcer models (mouse or rat) infected by S. aureus were selected as the research objects.

Results: The evaluation indicators were classified into four groups of criteria (including ten sub-indicators) and given different weights, physical sign changes (0.0518), skin lesion appearance (0.2934), morphological observation (0.3184), etiological examination (0.3364). Through the evaluation system, we screened and found that the mouse ulcer model which caused by a round wound and 1.0 × 10¹⁰ CFU/mL (0.1 mL) bacterial concentration got the highest comprehensive score, and also found that the model which caused by a 1.5 cm-round wound and 1.0 × 10¹⁰ CFU/mL (0.2 mL) maybe the best rat ulcer model.

Conclusions: This study has established an evaluation system based on AHP and Delphi method, also provided the best skin ulcer models selected by this system, the models are suitable for disease research and drug development research of skin ulcer.

Keywords: Analytic hierarchy process(AHP); Animal model; Delphi method; Evaluation index system; Skin infection.