A 3D-printed SERS bionic taster for dynamic tumor metabolites detection

Lei Wu; Lu Chen; Ziting Qian; Tingyu Wang; Qianqian Dong; Yizhi Zhang; Shenfei Zong; Yiping Cui; Zhuyuan Wang

doi:10.1016/j.talanta.2023.124766

A 3D-printed SERS bionic taster for dynamic tumor metabolites detection

Talanta. 2023 Nov 1:264:124766. doi: 10.1016/j.talanta.2023.124766. Epub 2023 Jun 3.

Authors

Lei Wu¹, Lu Chen¹, Ziting Qian¹, Tingyu Wang¹, Qianqian Dong¹, Yizhi Zhang², Shenfei Zong¹, Yiping Cui¹, Zhuyuan Wang³

Affiliations

¹ Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, 2 Sipailou, Nanjing 210096, China.
² Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, 29 Jiangjun Avenue, Nanjing 211106, China.
³ Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, 2 Sipailou, Nanjing 210096, China. Electronic address: wangzy@seu.edu.cn.

PMID: 37285698
DOI: 10.1016/j.talanta.2023.124766

Abstract

The variation of tumor-associated metabolites in extracellular microenvironment timely reflects the development, the progression and the treatment of cancers. Conventional methods for metabolite detection lack the efficiency to grasp the dynamic metabolic alterations. Herein, we developed a SERS bionic taster which enabled real-time analysis of extracellular metabolites. The instant information of cell metabolism was provided by the responsive Raman reporters, which experienced SERS spectral changes upon metabolite activation. Such a SERS sensor was integrated into a 3D-printed fixture which fits the commercial-standard cell culture dishes, allowing in-situ acquisition of the vibrational spectrum. The SERS taster can not only accomplish simultaneous and quantitative analysis of multiple tumor-associated metabolites, but also fulfill the dynamic monitoring of cellular metabolic reprogramming, which is expected to become a promising tool for investigating cancer biology and therapeutics.

Keywords: 3D printing; Bionic taster; SERS; Tumor metabolites.

MeSH terms

Bionics
Metal Nanoparticles*
Printing, Three-Dimensional
Spectrum Analysis, Raman / methods