Genetic Code Expansion in Mammalian Cells

Zhigang Wu; Jie Wang

doi:10.1007/978-1-0716-3251-2_11

Genetic Code Expansion in Mammalian Cells

Methods Mol Biol. 2023:2676:159-167. doi: 10.1007/978-1-0716-3251-2_11.

Authors

Zhigang Wu¹, Jie Wang²

Affiliations

¹ Department of Chemistry, Southern University of Science and Technology, Shenzhen, China.
² Department of Chemistry, Southern University of Science and Technology, Shenzhen, China. wangjie@sustech.edu.cn.

PMID: 37277631
DOI: 10.1007/978-1-0716-3251-2_11

Abstract

The expansion of the genetic code has enabled the incorporation of noncanonical amino acids (ncAAs) into a defined site of proteins. By introducing such a unique handle into the protein of interest (POI), bioorthogonal reactions can be utilized in live cells to monitor or manipulate the interaction, translocation, function, and modification of the POI. Here, we describe a basic protocol outlining the necessary steps to incorporate a ncAA into a POI in mammalian cells.

Keywords: Cell transfection; Genetic code expansion; Mammalian cells; Non-canonical amino acids; Stable cell line.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / chemistry
Amino Acyl-tRNA Synthetases* / metabolism
Animals
Genetic Code
Mammals / genetics
Mammals / metabolism
Proteins* / chemistry

Substances

Proteins
Amino Acids
Amino Acyl-tRNA Synthetases