We measured acute toxicity of triclosan, caffeine, nanoplastics, and microplastics, and their mixtures on Daphnia magna. Limitations of this study included use of a single species, acute rather than chronic toxicity testing, examination of single substances and their mixtures, and laboratory conditions that may not reflect real-world scenarios. Single compound toxicity results revealed a clear concentration-response pattern, with triclosan showing higher toxicity than caffeine, and nanoplastics displaying higher toxicity than microplastics. Combinations of triclosan with nanoplastics, and microplastics resulted in varying mortality rates, with higher rates observed with increased concentrations of triclosan and nanoplastics. Similar results were observed with caffeine, nanoplastics, and microplastics mixtures. These findings underline potential hazards posed by these pollutants to marine ecosystems and highlight the need for further studies to understand chronic effects, interactive effects of multiple substances, and the impact under more environmentally relevant conditions.
Keywords: Antagonistic; Emerging pollutants; Environmental impact; Environmental stressors; Single/mixture toxicity; Synergistic.
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