This study evaluated the effect of tricalcium phosphate-containing fluoride varnish on the prevention of root caries using an in-air micro-proton induced X-ray/gamma-ray emission system and microcomputed tomography. Either fluoride varnish (FV) or tricalcium phosphate-containing fluoride varnish (WV) was applied to root dentin, whereas dentin without varnish were considered controls. After immersion in saline, dentin sections were prepared, and concentration of fluoride ion was measured. These specimens were demineralized, and the mineral loss was measured. Caries inhibition in sound and demineralized dentin was observed in both FV and WV groups compared to the control group. Significantly low mineral loss was found especially in the demineralized dentin, which is probably due to the combination effect of calcium and fluoride ions at a concentration range of 100,000 ppm, as supplied by the varnish. The use of calcium-containing fluoride varnish, especially in demineralized dentin, is effective in arresting initial dentin caries.
Keywords: Fluoride; PIXE/PIGE; Root caries; Tricalcium phosphate.