Identification of the matrix metalloproteinase (MMP) gene family in Japanese flounder (Paralichthys olivaceus): Involved in immune response regulation to temperature stress and Edwardsiella tarda infection

Huihui Xie; Jiabao Hu; Yajun Wang; Xubo Wang

doi:10.1016/j.fsi.2023.108878

Identification of the matrix metalloproteinase (MMP) gene family in Japanese flounder (Paralichthys olivaceus): Involved in immune response regulation to temperature stress and Edwardsiella tarda infection

Fish Shellfish Immunol. 2023 Aug:139:108878. doi: 10.1016/j.fsi.2023.108878. Epub 2023 Jun 2.

Authors

Huihui Xie¹, Jiabao Hu², Yajun Wang³, Xubo Wang⁴

Affiliations

¹ National Engineering Research Laboratory of Marine Biotechnology and Engineering, Ningbo University, China; Key Laboratory of Aquacultural Biotechnology (Ningbo University), Ministry of Education, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction By Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, China.
² National Engineering Research Laboratory of Marine Biotechnology and Engineering, Ningbo University, China; Key Laboratory of Aquacultural Biotechnology (Ningbo University), Ministry of Education, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction By Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, China; School of Civil & Environmental Engineering and Geography Science, Ningbo University, Ningbo, China.
³ National Engineering Research Laboratory of Marine Biotechnology and Engineering, Ningbo University, China; Key Laboratory of Aquacultural Biotechnology (Ningbo University), Ministry of Education, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction By Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, China. Electronic address: wangyajun@nbu.edu.cn.
⁴ National Engineering Research Laboratory of Marine Biotechnology and Engineering, Ningbo University, China; Key Laboratory of Aquacultural Biotechnology (Ningbo University), Ministry of Education, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction By Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, China. Electronic address: wangxubo@nbu.edu.cn.

PMID: 37271328
DOI: 10.1016/j.fsi.2023.108878

Abstract

The Matrix metalloproteinase (MMP) gene family is responsible for regulating the degradation of Extra Cellular Matrix (ECM) proteins, which are important for physiological processes such as wound healing, tissue remodeling, and stress response. Although MMPs have been studied in many species, their role in immune response in Japanese flounder (Paralichthys olivaceus) is still not fully understood. This study conducted a comprehensive analysis of MMPs in flounder, including gene structures, evolutionary relationships, conserved domains, molecular evolution, and expression patterns. Analysis revealed that MMP genes could be grouped into 17 subfamilies and were evolutionarily conserved and functionally-constrained. Meanwhile, MMP genes were found to express in different embryonic and larval stages and might play the role of sentinel in healthy tissues. Furthermore, expression profiling showed that MMPs had diverse functions in environmental stress, with 60% (9/15) and 73% (11/15) of MMPs showing differential expression patterns under temperature stress and Edwardsiella tarda (E. tarda) infection, respectively. These findings provide a useful resource for understanding the immune functions of MMP genes in Japanese flounder.

Keywords: Edwardsiella tarda infection; Japanese flounder; MMP gene Family; Temperature stress.

MeSH terms

Animals
Edwardsiella tarda / physiology
Enterobacteriaceae Infections* / veterinary
Fish Diseases*
Flounder* / genetics
Immunity
Matrix Metalloproteinases / genetics
Temperature

Substances

Matrix Metalloproteinases