ICESpsuAH0906, a novel optrA-carrying element conferring resistance to phenicols and oxazolidinones from Streptococcus parasuis, is transferable to Streptococcus suis

Xingyang Dai; Jiaqi Zhao; Junjie Sun; Li Chen; Peizhao Han; Xiaoming Wang; Jinhu Huang; Liping Wang

doi:10.1016/j.vetmic.2023.109795

ICESpsuAH0906, a novel optrA-carrying element conferring resistance to phenicols and oxazolidinones from Streptococcus parasuis, is transferable to Streptococcus suis

Vet Microbiol. 2023 Aug:283:109795. doi: 10.1016/j.vetmic.2023.109795. Epub 2023 May 30.

Authors

Xingyang Dai¹, Jiaqi Zhao¹, Junjie Sun¹, Li Chen¹, Peizhao Han¹, Xiaoming Wang², Jinhu Huang³, Liping Wang⁴

Affiliations

¹ MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
² MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Risk Assessment Center of Veterinary Drug Residue and Antimicrobial Resistance, Nanjing Agricultural University, Nanjing 210095, China; Center for Veterinary Drug Research and Evaluation, Nanjing Agricultural University, Nanjing 210095, China.
³ MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Risk Assessment Center of Veterinary Drug Residue and Antimicrobial Resistance, Nanjing Agricultural University, Nanjing 210095, China; Center for Veterinary Drug Research and Evaluation, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: jhuang@njau.edu.cn.
⁴ MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Risk Assessment Center of Veterinary Drug Residue and Antimicrobial Resistance, Nanjing Agricultural University, Nanjing 210095, China; Center for Veterinary Drug Research and Evaluation, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wlp71@163.com.

PMID: 37269713
DOI: 10.1016/j.vetmic.2023.109795

Abstract

Streptococcus parasuis is a potential opportunistic zoonotic pathogen which is a close relative to Streptococcus suis, which exhibit extensive genetic exchange. The occurrence and dissemination of oxazolidinone resistance poses a severe threat to public health. However, such knowledge about the optrA gene in S. parasuis is limited. Herein, we characterized an optrA-positive multi-resistant S. parasuis isolate AH0906, in which the capsular polysaccharide locus exhibited a hybrid structure of S. suis serotype 11 and S. parasuis serotype 26. The optrA and erm(B) genes were co-located on a novel ICE of the ICESsuYZDH1 family, designated ICESpsuAH0906. IS1216E-optrA-carrying translocatable unit could be formed when excised from ICESpsuAH0906. ICESpsuAH0906 was found to be transferable from isolate AH0906 to Streptococcus suis P1/7RF at a relative high frequency of ∼ 10^-5. Nonconservative integrations of ICESpsuAH0906 into the primary site SSU0877 and secondary site SSU1797 with 2-/4-nt imperfect direct repeats in recipient P1/7RF were observed. Upon transfer, the transconjugant displayed elevated MICs of the corresponding antimicrobial agents and performed a weak fitness cost when compared with the recipient strain. To our knowledge, it is the first description of the transfer of optrA in S. prarasuis and the first report of interspecies transfer of ICE with triplet serine integrases (of the ICESsuYZDH1 family). Considering the high transmission frequency of the ICEs and the extensive genetic exchange potential of S. parasuis with other streptococci, attention should be paid to the dissemination of the optrA gene from S. parasuis to clinically more important bacterial pathogens.

Keywords: Horizontal gene transfer; ICESsuYZDH1 family; Integrative and conjugative elements; Streptococcus parasuis; optrA gene.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Anti-Infective Agents* / pharmacology
Drug Resistance, Bacterial / genetics
Genes, Bacterial
Oxazolidinones*
Streptococcus suis*

Substances

Oxazolidinones
Anti-Infective Agents
Anti-Bacterial Agents

Supplementary concepts

Streptococcus parasuis