A hydrogel-based first-aid tissue adhesive with effective hemostasis and anti-bacteria for trauma emergency management

Dongjie Zhang; Li Mei; Yuanping Hao; Bingcheng Yi; Jilin Hu; Danyang Wang; Yaodong Zhao; Zhe Wang; Hailin Huang; Yongzhi Xu; Xuyang Deng; Cong Li; Xuewei Li; Qihui Zhou; Yun Lu

doi:10.1186/s40824-023-00392-9

A hydrogel-based first-aid tissue adhesive with effective hemostasis and anti-bacteria for trauma emergency management

Biomater Res. 2023 Jun 2;27(1):56. doi: 10.1186/s40824-023-00392-9.

Authors

Dongjie Zhang¹, Li Mei², Yuanping Hao³, Bingcheng Yi⁴, Jilin Hu¹, Danyang Wang², Yaodong Zhao^{1

2}, Zhe Wang², Hailin Huang², Yongzhi Xu³, Xuyang Deng², Cong Li², Xuewei Li⁵, Qihui Zhou^{6

7}, Yun Lu⁸

Affiliations

¹ Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.
² Department of Stomatology, Qingdao University, Qingdao, 266021, China.
³ Department of Stomatology, Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, 266003, China.
⁴ School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao, 266071, China.
⁵ Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.
⁶ School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao, 266071, China. qihuizhou@uor.edu.cn.
⁷ Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325000, Zhejiang, China. qihuizhou@uor.edu.cn.
⁸ Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China. luyun@qdu.edu.cn.

Abstract

Background: Clinical tissue adhesives remain some critical drawbacks for managing emergency injuries, such as inadequate adhesive strength and insufficient anti-infection ability. Herein, a novel, self-healing, and antibacterial carboxymethyl chitosan/polyaldehyde dextran (CMCS/PD) hydrogel is designed as the first-aid tissue adhesive for effective trauma emergency management.

Methods: We examined the gel-forming time, porosity, self-healing, antibacterial properties, cytotoxicity, adhesive strength, and hemocompatibility. Liver hemorrhage, tail severance, and skin wound infection models of rats are constructed in vivo, respectively.

Results: Results demonstrate that the CMCS/PD hydrogel has the rapid gel-forming (~ 5 s), good self-healing, and effective antibacterial abilities, and could adhere to tissue firmly (adhesive strength of ~ 10 kPa and burst pressure of 327.5 mmHg) with excellent hemocompatibility and cytocompatibility. This suggests the great prospect of CMCS/PD hydrogel in acting as a first-aid tissue adhesive for trauma emergency management. The CMCS/PD hydrogel is observed to not only achieve rapid hemostasis for curing liver hemorrhage and tail severance in comparison to commercial hemostatic gel (Surgiflo ®) but also exhibit superior anti-infection for treating acute skin trauma compared with clinical disinfectant gel (Prontosan ®).

Conclusions: Overall, the CMCS/PD hydrogel offers a promising candidate for first-aid tissue adhesives to manage the trauma emergency. Because of the rapid gel-forming time, it could also be applied as a liquid first-aid bandage for mini-invasive surgical treatment.

Keywords: Anti-infection; Carboxymethyl chitosan; Hydrogel; Self-healing; Tissue adhesive; Trauma emergency.

Abstract

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