3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy

Congsun Li; Jie Wang; Weinan Yang; Kang Yu; Jianqiao Hong; Xiaoxiao Ji; Minjun Yao; Sihao Li; Jinwei Lu; Yazhou Chen; Shigui Yan; Haobo Wu; Chiyuan Ma; Xiaohua Yu; Guangyao Jiang; An Liu

doi:10.1186/s12951-023-01892-5

3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy

J Nanobiotechnology. 2023 Jun 3;21(1):177. doi: 10.1186/s12951-023-01892-5.

Authors

Congsun Li^#^{1

2

3

4}, Jie Wang^#^{1

2

3

4}, Weinan Yang^#^{1

2

3

4}, Kang Yu⁵, Jianqiao Hong^{1

2

3

4}, Xiaoxiao Ji¹, Minjun Yao^{1

2

3

4}, Sihao Li^{1

2

3

4}, Jinwei Lu^{1

2

3

4}, Yazhou Chen^{1

2

3

4}, Shigui Yan^{1

2

3

4}, Haobo Wu^{1

2

3

4}, Chiyuan Ma^{1

2

3

4}, Xiaohua Yu^{6

7

8

9}, Guangyao Jiang^{10

11

12

13}, An Liu^{14

15

16

17}

Affiliations

¹ Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China.
² Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China.
³ Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China.
⁴ Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, PR China.
⁵ State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Hangzhou, Zhejiang, PR China.
⁶ Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China. xiaohua.yu@zju.edu.cn.
⁷ Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China. xiaohua.yu@zju.edu.cn.
⁸ Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China. xiaohua.yu@zju.edu.cn.
⁹ Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, PR China. xiaohua.yu@zju.edu.cn.
¹⁰ Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China. 11718243@zju.edu.cn.
¹¹ Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China. 11718243@zju.edu.cn.
¹² Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China. 11718243@zju.edu.cn.
¹³ Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, PR China. 11718243@zju.edu.cn.
¹⁴ Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China. la@zju.edu.cn.
¹⁵ Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China. la@zju.edu.cn.
¹⁶ Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China. la@zju.edu.cn.
¹⁷ Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, PR China. la@zju.edu.cn.

^# Contributed equally.

Abstract

Long-term chronic inflammation after Achilles tendon injury is critical for tendinopathy. Platelet-rich plasma (PRP) injection, which is a common method for treating tendinopathy, has positive effects on tendon repair. In addition, tendon-derived stem cells (TDSCs), which are stem cells located in tendons, play a major role in maintaining tissue homeostasis and postinjury repair. In this study, injectable gelatine methacryloyl (GelMA) microparticles containing PRP laden with TDSCs (PRP-TDSC-GM) were prepared by a projection-based 3D bioprinting technique. Our results showed that PRP-TDSC-GM could promote tendon differentiation in TDSCs and reduce the inflammatory response by downregulating the PI3K-AKT pathway, thus promoting the structural and functional repair of tendons in vivo.

Keywords: Hydrogel; Inflammation; PI3K-AKT pathway; TDSCs; Tendinopathy.

MeSH terms

Animals
Hydrogels / pharmacology
Phosphatidylinositol 3-Kinases / metabolism
Platelet-Rich Plasma* / metabolism
Printing, Three-Dimensional
Rats
Stem Cells
Tendinopathy* / metabolism
Tendinopathy* / therapy
Tendons

Substances

Hydrogels
Phosphatidylinositol 3-Kinases

Abstract

MeSH terms

Substances

Grants and funding