Three new furanones from endophytic fungus Hypoxylon vinosopulvinatum DYR-1-7 from Cinnamomum cassia with their antifungal activity

Jia Lv; Hong Zhou; Limei Dong; Hao Wang; Li Yang; Haiqian Yu; Peixi Wu; Le Zhou; Quan Yang; Yongqian Liang; Bi Luo

doi:10.1080/14786419.2023.2218530

Three new furanones from endophytic fungus Hypoxylon vinosopulvinatum DYR-1-7 from Cinnamomum cassia with their antifungal activity

Nat Prod Res. 2023 Jun 2:1-8. doi: 10.1080/14786419.2023.2218530. Online ahead of print.

Authors

Jia Lv^{1

2

3}, Hong Zhou^{4

5}, Limei Dong⁶, Hao Wang⁷, Li Yang⁷, Haiqian Yu⁸, Peixi Wu⁹, Le Zhou¹⁰, Quan Yang^{1

2}, Yongqian Liang⁹, Bi Luo^{1

2}

Affiliations

¹ School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China.
² Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials, Guangzhou, Guangdong, China.
³ School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
⁴ National Clinical Research Center for Kidney Disease, Nanfang Hospital, Guangzhou, Guangdong, China.
⁵ Guangdong Provincial Clinical Research Center for Kidney Disease, Guangzhou, Guangdong, China.
⁶ Guangdong Eco-Engineering Polytechnic, Guangzhou, Guangdong, China.
⁷ Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
⁸ Lei Yun Shang Pharmaceutical Group Co., Ltd, Suzhou, Jiangsu, China.
⁹ School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China.
¹⁰ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Chinese Academy of Sceinces, South China Sea Institute of Oceanology, Guangdong, Guangzhou, China.

PMID: 37267595
DOI: 10.1080/14786419.2023.2218530

Abstract

Hypoxylon vinosopulvinatum DYR-1-7 is a endophytic fungus isolated from the Cinnamomum cassia Presl and has an inhibitory effect on Lasiodiplodia pseudotheobromae. Three new furanones, hypoxylonone A-C (1-3), as well as three known compounds (4-6), were isolated from an EtOAc extract of H. vinosopulvinatum DYR-1-7. The structures were determined by spectroscopic data analysis using UV, IR, 1D-, 2D-NMR and HR-ESI-MS. The absolute configurations of 1-3 were elucidated by electronic circular dichroism (ECD) analyses. In the antifungal bioassay, Hypoxylonone B and C exhibited strong inhibitory effects on L. pseudotheobromae with IC₅₀ value at the concentration of 1.01 and 2.40 μg/mL, respectively. Compound 6 showed medium antifungal activity with IC₅₀ value at the concentration of 10.67 μg/mL on Fusarium oxysporum. Compounds 3 and 4 displayed medium antifungul effects on Candida albicans.

Keywords: Cinnamomum cassia; Hypoxylon vinosopulvinatum;; antifungal activity; endophytic fungus; secondary metabolites.