Pharmacokinetic study about compatibility of Eucommia ulmoides and Psoralea corylifolia

Yuxing Huang; Erwei Liu; Xuhua Huang; Jia Hao; Siyuan Hu; Xiumei Gao

doi:10.1016/j.chmed.2022.09.005

Pharmacokinetic study about compatibility of Eucommia ulmoides and Psoralea corylifolia

Chin Herb Med. 2023 Mar 15;15(2):263-270. doi: 10.1016/j.chmed.2022.09.005. eCollection 2023 Apr.

Authors

Yuxing Huang^{1

2

3}, Erwei Liu^{1

4}, Xuhua Huang¹, Jia Hao^{1

4}, Siyuan Hu², Xiumei Gao^{1

5}

Affiliations

¹ Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
² First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
³ National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China.
⁴ Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
⁵ Tianjin Key Laboratory of Chinese Medicine Pharmocology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Abstract

Objective: The compatibility of Eucommia ulmoides (Eu) and Psoralea corylifolia (Pc) on the pharmacokinetic (PK) properties in the rat was explored in this study.

Methods: Eu extract, Pc extract and the combined extracts (crude drug ratio was 2:1) was administered by gavage, respectively. Two PK experiments were conducted. In first one, the blood samples were collected via the occuli chorioideae vein to get the PK properties of the components. In second one, the blood samples were simultaneously collected via the internal jugular vein or portal vein at different time points and the concentrations of target ingredients were detected by LC/MS/MS to clear the location where the interaction of Eu and Pc took place in vivo.

Results: Eight of 11 ingredients in Eu and Pc extract were determined in rat plasma. The exposure levels of geniposidic acid (GPA), aucubin (AU), geniposide (GP), pinoresinol diglucoside (PDG), psoralen glycosides (PLG) and isopsoralen glycosides (IPLG) were decreased 1/5-2/3 after administration of combined extracts. Comparing to the combined administration, the exposure of GPA and AU in plasma of single Eu administration collected via the portal vein were decreased 1/3-2/3, and the values of AUC_0-24h and AUC_0-∞ of GP collected from the portal vein or internal jugular vein were double increased. The other components' parameters were not significantly changed.

Conclusion: In summary, the Pc and Eu combined administration could affect the exposure of the main components of Eu extract in rats due to the changed intestinal absorption. The research on the compatibility of Pc and Eu was helpful to guide the clinical administration of Eu and Pc simultaneously.

Keywords: Eucommia ulmoides Oliver; LC/MS/MS; Psoralea corylifolia L; double intubation; drug interaction; pharmacokinetic properties.