Open riparian canopy and nutrient pollution interactively decrease trophic redundancy and allochthonous resource in streams

Abstract

Riparian deforestation, which leads to increase in light intensity and excessive nutrient loading in waterways, are two pervasive environmental stressors in the stream ecosystems. Both have been found to alter basal resource availability and consequently stream food webs. However, their interactive effects on trophic structure in stream food webs are unclear. Here, we manipulated light intensity and nutrient availability in three headwater streams to evaluate their effects on consumer diet composition and food web characteristics (i.e., trophic diversity and redundancy) with stable isotope analysis. Dietary analysis revealed that the relative contribution of stream periphyton to the diets of macroinvertebrates increased, while that of allochthonous resources, specifically leaf litter from the terrestrial ecosystems in the catchment, decreased in response to open canopy and nutrient enrichment in the streams. The trophic diversity also increased with the elevated light intensity and nutrient availability, while the trophic redundancy decreased, suggesting a reduced ability of the stream ecosystems to resist environmental changes. Nutrient enrichment also increased the δ¹⁵N ratios of periphyton and macroinvertebrates, indicating potential δ¹⁵N enrichment of stream benthos by nitrogen pollution. Our results suggested that an increase in light intensity due to riparian canopy openness and stream water nutrient enrichment primarily from human activities have interactive effects on resource flow and trophic structure in stream food webs.

Keywords: Diet estimation; Ecosystem function; Ecosystem structure; Isotopic metrics; Macroinvertebrates; Stable isotopes.