Ozone membrane contactor for tertiary treatment of urban wastewater: Chemical, microbial and toxicological assessment

Abstract

A membrane ozone contactor, operated under continuous mode, was applied to promote the tertiary treatment of urban wastewater (UWW), targeting the removal of contaminants of emerging concern (CECs), bacterial disinfection, and toxicity reduction. This system relies on the homogeneous radial distribution of ozone (O₃) in the reaction zone by "titration" through a microfiltration borosilicate tubular membrane, while the UWW swirls around the membrane and drags the O₃ microbubbles generated in the membrane shell-side. The membrane is coated with titanium dioxide (TiO₂-P25) and radiation can be externally supplied via four UV lamps. The ozonation tests were carried out with secondary-treated UWW collected in different seasons (winter and summer) and spiked with a mix of 19 CECs (10 μg L^-1 each). For an O₃ dose of 18 g m^-3, the best performance was obtained by increasing the O₃ concentration (maximum [O₃]_G,inlet of 200 g Nm^-3) and decreasing the gas flow rate (minimum Q_G of 0.15 Ndm³ min^-1), providing the highest ozone transfer yield (88 %) and, thus higher specific ozone dose (g O₃ per g dissolved organic carbon). Under these conditions, removals >80 % or concentrations below the limit of quantification were obtained for up to 13 of the 19 CECs and reductions up to 5 log units for total heterotrophs and below the limit of detection for enterobacteria and enterococci. Tests including a UVC dose of 0.10 kJ L^-1 enhanced disinfection ability but had no impact on CECs oxidation. After ozonation, the abundance of antibiotic resistant bacteria was reduced but not eliminated, and microbial regrowth after 3-day storage was observed. No toxic effect was detected on zebrafish embryos using a dilution factor of 4 for the ozonized UWW and when granular activated carbon adsorption was subsequently applied the dilution factor decreased to 2.

Keywords: Contaminants of emerging concern; Disinfection; Ozonation; Ozone membrane contactor; Zebrafish embryos bioassays.