Influence of age and gender on alveolar bone healing post tooth extraction in 129 Sv mice: a microtomographic, histological, and biochemical characterization

Claudia Cristina Biguetti; Bhuvana Lakkasetter Chandrashekar; Gustavo Baroni Simionato; Nataira Regina Momesso; Marco Antonio Hungaro Duarte; Danieli C Rodrigues; Mariza Akemi Matsumoto

doi:10.1007/s00784-023-05087-y

Influence of age and gender on alveolar bone healing post tooth extraction in 129 Sv mice: a microtomographic, histological, and biochemical characterization

Clin Oral Investig. 2023 Aug;27(8):4605-4616. doi: 10.1007/s00784-023-05087-y. Epub 2023 Jun 1.

Authors

Claudia Cristina Biguetti^{1

2}, Bhuvana Lakkasetter Chandrashekar³, Gustavo Baroni Simionato⁴, Nataira Regina Momesso⁴, Marco Antonio Hungaro Duarte⁵, Danieli C Rodrigues³, Mariza Akemi Matsumoto⁴

Affiliations

¹ Department of Surgery and Biomechanics, School of Podiatric Medicine, University of Texas Rio Grande Valley, Harlingen, TX, USA. claudia.biguetti@utrgv.edu.
² Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), São Paulo, Araçatuba, Brazil. claudia.biguetti@utrgv.edu.
³ Department of Bioengineering, University of at Dallas, Richardson, TX, USA.
⁴ Department of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), São Paulo, Araçatuba, Brazil.
⁵ Department of Dental Materials and Endodontics, School of Dentistry of Bauru, University of Sao Paulo (USP), Bauru, São Paulo, Brazil.

PMID: 37261497
DOI: 10.1007/s00784-023-05087-y

Abstract

Objectives: To analyze the effect of biological sex and aging on craniofacial bone features in 129 Sv mice and their influence on dental socket healing post tooth extraction.

Materials and methods: A total of 52 129 Sv mice were used, of which 28 were young (3-4 months) and 24 were aged (17-18 months), equally distributed according to biological sex. After an upper right incisor extraction, mice specimens were collected at 7, 14, and 21-days post-surgery for microtomographic (microCT) and comprehensive histological analysis. Mandible, skull bones, and maxillae at 21 days were analyzed by microCT, while blood plasma samples were collected for the detection of key bone turnover markers (P1NP and CTX-1) by enzyme-linked immunosorbent (ELISA) assay.

Results: Aged females depicted significantly decreased mineralized bone content in alveolar sockets in comparison to young females and aged males at day 7, and aged males at day 14. Mandible RCA and Ma.AR of aged females were also significantly decreased in comparison with young females. Histological evaluation revealed that all alveolar sockets healed at 21 days with inflammation resolution and deposition of new bone. Immunohistochemistry for TRAP revealed increased area density for osteoclasts in alveolar sockets of aged females when compared to young females at 21 days. While a significant increase in CTX-1 levels was detected in blood plasma of aged females when compared to young females, P1NP levels did not significantly change between young and older females. No significant changes were observed for males.

Conclusions: Age and gender can significantly affect craniofacial bones of 129 Sv mice, especially maxilla and mandible in females. Considering the altered bone resorption parameters and delayed alveolar bone healing in older females, careful deliberation is necessary during development of pre-clinical models for craniofacial research.

Clinical relevance: Aging can be a contributing factor to slower bone healing in craniofacial bones. However, there are no sufficient experimental studies that have addressed this phenomenon along with biological sex taken into consideration.

Keywords: Aging; Bone tissue; Mice; Sexual dimorphism; Wound healing.

MeSH terms

Animals
Bone Resorption* / pathology
Dental Care
Female
Humans
Male
Mice
Periodontal Ligament
Tooth Extraction / methods
Tooth Socket* / diagnostic imaging
Tooth Socket* / pathology
Tooth Socket* / surgery

Abstract

MeSH terms

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