Appearance of ghost bands in gel electrophoresis depending on agarose concentration

Abstract

Standard agarose gel electrophoresis is a widely used method to analyse diversity of nucleic acids. Certain conditions, however, may give rise to artefactual bands. We report on artefactual bands frequently occurring, especially when partially homologous nucleic acids, such as splicing variants of DNA transcripts, are analysed simultaneously. Interestingly, to some extent agarose concentration may influence the occurrence of artefactual bands.

Keywords: CD33; CYP1A1; DNA splicing variant; artefactual band; ghost band.