Isolation and Purification of Mycobacterial Extracellular Vesicles (EVs)

Komal Umashankar Rao; Gabriela Godaly

doi:10.1007/978-1-0716-3243-7_3

Isolation and Purification of Mycobacterial Extracellular Vesicles (EVs)

Methods Mol Biol. 2023:2674:55-60. doi: 10.1007/978-1-0716-3243-7_3.

Authors

Komal Umashankar Rao¹, Gabriela Godaly²

Affiliations

¹ Department of Microbiology, Immunology and Glycobiology, Institution of Laboratory Medicine, Lund University, Lund, Sweden.
² Department of Microbiology, Immunology and Glycobiology, Institution of Laboratory Medicine, Lund University, Lund, Sweden. gabriela.godaly@med.lu.se.

PMID: 37258959
DOI: 10.1007/978-1-0716-3243-7_3

Abstract

Bacterial extracellular vesicles (EVs) contain numerous active substances that mediate bacterial interactions with their host and with other microbes. Best defined are the EVs from Gram-negative bacteria that have been shown to deliver virulence factors, modulate the immune responses, mediate antibiotic resistance, and also inhibit competitive microbes. Due to the complex cell wall structures of Gram-positive bacteria and mycobacteria, EVs from these bacteria were only recently reported. This protocol describes the isolation of EVs from mycobacteria.

Keywords: Extracellular vesicles; Mycobacterium tuberculosis; Vesicle isolation.

MeSH terms

Extracellular Vesicles* / chemistry
Gram-Positive Bacteria
Mycobacterium* / physiology
Virulence Factors / analysis

Substances

Virulence Factors