Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation

Dilem Shakir; Michael Batie; Sonia Rocha

doi:10.1007/978-1-0716-3247-5_19

Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation

Methods Mol Biol. 2023:2675:261-269. doi: 10.1007/978-1-0716-3247-5_19.

Authors

Dilem Shakir¹, Michael Batie¹, Sonia Rocha²

Affiliations

¹ Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
² Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK. Sonia.Rocha@liverpool.ac.uk.

PMID: 37258769
DOI: 10.1007/978-1-0716-3247-5_19

Abstract

Hypoxia and its signalling pathway play a key role in human physiology and a variety of diseases. Alterations in histone methylation coordinate transcriptional responses to hypoxia. Here, we detail a fixed cell immunofluorescence method for quantifying hypoxia-induced changes in histone methylation, exemplified by the measurement of H3K27me3.

Keywords: Histone methylation; Hypoxia; Immunofluorescence; Immunostaining.

MeSH terms

DNA Methylation
Fluorescent Antibody Technique
Histones* / metabolism
Humans
Hypoxia* / metabolism
Methylation
Protein Processing, Post-Translational

Substances

Histones