Metabolite Analyses Using Nuclear Magnetic Resonance (NMR) Spectroscopy in Plasma of Patients with Prostate Cancer

Dalia Ahmed; Stefano Cacciatore; Luiz Fernando Zerbini

doi:10.1007/978-1-0716-3247-5_15

Metabolite Analyses Using Nuclear Magnetic Resonance (NMR) Spectroscopy in Plasma of Patients with Prostate Cancer

Methods Mol Biol. 2023:2675:195-204. doi: 10.1007/978-1-0716-3247-5_15.

Authors

Dalia Ahmed^{1

2

3}, Stefano Cacciatore¹, Luiz Fernando Zerbini⁴

Affiliations

¹ Bioinformatics Unit, International Centre for Genetic Engineering and Biotechnology, Cape Town, South Africa.
² Faculty of Medical Laboratory Science, Department of Histopathology and Cytology, IBN SINA University, Khartoum, Sudan.
³ Faculty of Medical Laboratory Science, Department of Histopathology and Cytology, Omdurman Ahlia University, Omdurman, Sudan.
⁴ Cancer Genomics Group, International Centre for Genetic Engineering and Biotechnology, Cape Town, South Africa. luiz.zerbini@icgeb.org.

PMID: 37258765
DOI: 10.1007/978-1-0716-3247-5_15

Abstract

Nuclear magnetic resonance (NMR) spectroscopy enables the detection and the quantification of a large range of molecules, including low-molecular-weight metabolites and lipids. NMR spectroscopy is a powerful approach when applied to the high-throughput analysis of plasma or serum samples allowing, in addition, the detection of total proteins, lipoproteins, and signals arising from the glycosylation of circulating acute-phase proteins. Here, we describe the usage of NMR spectroscopy for profiling the plasma or serum of patients with prostate cancer.

Keywords: Metabolic profiling; Metabolomics; NMR; Prostate cancer; Standard operating procedures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Magnetic Resonance Imaging
Magnetic Resonance Spectroscopy / methods
Male
Metabolomics* / methods
Plasma / metabolism
Prostatic Neoplasms* / metabolism