Isolation of Mitochondria from Mouse Tissues for Functional Analysis

Rebeca Acín-Pérez; Katrina P Montales; Kaitlyn B Nguyen; Alexandra J Brownstein; Linsey Stiles; Ajit S Divakaruni

doi:10.1007/978-1-0716-3247-5_7

Isolation of Mitochondria from Mouse Tissues for Functional Analysis

Methods Mol Biol. 2023:2675:77-96. doi: 10.1007/978-1-0716-3247-5_7.

Authors

Rebeca Acín-Pérez¹, Katrina P Montales¹, Kaitlyn B Nguyen², Alexandra J Brownstein¹, Linsey Stiles^{1

2}, Ajit S Divakaruni³

Affiliations

¹ Department of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
² Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA.
³ Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA. adivakaruni@mednet.ucla.edu.

Abstract

Methods for isolating mitochondria from different rodent tissues have been established for decades. Although the general principles for crude mitochondrial preparations are largely shared across tissues - tissue disruption followed by differential centrifugation - critical differences exist for isolation from different tissues to optimize mitochondrial yield and function. This protocol offers a unified resource for preparations of isolated mitochondria from mouse liver, kidney, heart, brain, skeletal muscle, and brown and white adipose tissue suitable for functional analysis.

Keywords: Bioenergetics; Brain; Brown adipose tissue; Heart; Kidney; Liver; Mitochondria; Oxidative phosphorylation; Skeletal muscle; White adipose tissue.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Adipose Tissue, White* / metabolism
Animals
Mice
Mitochondria*
Mitochondria, Muscle / metabolism
Muscle, Skeletal / metabolism

Grants and funding

R35 GM138003/GM/NIGMS NIH HHS/United States