Effect of Bitter-leaf (Vernonia amygdalina) Flavored Non-alcoholic Wheat Beer (NAWB) on, Insulin and GLUT-2 expression in Pancreas of High fat diet/Streptozotocin (HFD/STZ) Induced Diabetic Wistar Rats

Gbenga P Akerele; Bukola C Adedayo; Ganiyu Oboh; Opeyemi B Ogunsuyi

doi:10.1007/s40200-023-01216-2

Effect of Bitter-leaf (Vernonia amygdalina) Flavored Non-alcoholic Wheat Beer (NAWB) on, Insulin and GLUT-2 expression in Pancreas of High fat diet/Streptozotocin (HFD/STZ) Induced Diabetic Wistar Rats

J Diabetes Metab Disord. 2023 Mar 25;22(1):873-880. doi: 10.1007/s40200-023-01216-2. eCollection 2023 Jun.

Authors

Gbenga P Akerele¹, Bukola C Adedayo¹, Ganiyu Oboh¹, Opeyemi B Ogunsuyi^{1

2}

Affiliations

¹ Functional Foods and Nutraceutical Unit, Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, Akure, , 340,001 Nigeria.
² Department of Biomedical Technology, Federal University of Technology, Akure, P.M.P. 704, Akure, 340,001 Nigeria.

Abstract

Purpose: In the management of type 2 diabetes (T2D), dietary intervention has been proposed to be highly effective. This study, therefore, investigated the effect of bitter leaf-flavored non-alcoholic wheat beer (NAWB) on insulin secretion and GLUT-2 expression in the pancreas of STZ-induced diabetic rats.

Methods: In this study, the rats received a single intraperitoneal injection (i.p.) of STZ (35 mg/kg) after being fed a high-fat diet for 14 days to induce T2D. The rats were treated with bitter leaf flavored NAWB samples (100%HP- Hops only, 100%BL-Bitter leaf only, 75,25BL- 75% Hops, 25% Bitter Leaf, 50:50BL- 50% Hops:50% Bitter Leaf, and 25:75BL-25%Hops:75% Bitter Leaf) and Acarbose for 14 days. The superoxide dismutase, catalase, and glutathione peroxidase activity were also determined.

Results: The results from this study showed a correlation between GLUT-2 and Insulin expression. There was an upregulation of Insulin as GLUT-2 expression was upregulated. Furthermore, the treated groups showed better antioxidant activity when compared with the diabetic control.

Conclusion: Bitter leaf-flavored NAWB might thus be a good dietary intervention for type 2 diabetics.

Keywords: Antioxidant; Diabetes Mellitus Type 2 (T2D); Glucose Transporter Type 2 (GLUT-2); Insulin; Non-alcoholic beer.

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