Novel antiosteoporotic peptides purified from protein hydrolysates of taihe black-boned silky fowl: By larval zebrafish model and molecular docking

Yang Qiu; Jianyue Ying; Fujie Yan; Huilin Yu; Yan Zhao; Honghao Li; Shengyao Xia; Jianchu Chen; Jiajin Zhu

doi:10.1016/j.foodres.2023.112850

Novel antiosteoporotic peptides purified from protein hydrolysates of taihe black-boned silky fowl: By larval zebrafish model and molecular docking

Food Res Int. 2023 Jul:169:112850. doi: 10.1016/j.foodres.2023.112850. Epub 2023 Apr 19.

Authors

Yang Qiu¹, Jianyue Ying², Fujie Yan¹, Huilin Yu¹, Yan Zhao¹, Honghao Li¹, Shengyao Xia¹, Jianchu Chen³, Jiajin Zhu⁴

Affiliations

¹ College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China.
² Zhejiang University Hospital, Hangzhou 310027, China.
³ College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: jc@zju.edu.cn.
⁴ College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Engineering Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China. Electronic address: jjzhu@zju.edu.cn.

PMID: 37254422
DOI: 10.1016/j.foodres.2023.112850

Abstract

The black-boned silky fowl (BSF) muscle protein hydrolysate was gained by alcalase. The hydrolysate could stimulate MC3T3-E1 cell proliferation, as well as enhance alkaline phosphatas (ALP) activity and deposits of minerals. After isolation and purification, 55 peptide sequences with Mascot score over 40 were identified. Combined with molecular docking simulation and molecular dynamics analysis, two novel peptides (PASTGAAK and PGPPGTPF) were identified with the lowest binding energy of -4.99 kcal/mol and -3.07 kcal/mol with receptor BMPR1A of BMP-2/Smad pathway, showing the ability to increase BMPR1A stability. Moreover, both PASTGAAK and PGPPGTPF revealed strong anti-osteoporosis activities in the zebrafish model induced by dexamethasone. Additionally, the identified peptides could be beneficial for the differentiation of MC3T3-E1 cell for upregulating the expression of some osteoblast-related genes and proteins by stimulating BMP-2/Smad pathway. Overall, the two newly identified peptides could be the potential candidate to prevent osteoporosis.

Keywords: Black-boned silky fowl; Osteoporosis; Purification; Screening; Zebrafish.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens
Larva
Molecular Docking Simulation
Peptides / pharmacology
Protein Hydrolysates* / pharmacology
Zebrafish*

Substances

Peptides
Protein Hydrolysates