A Route to Synthesize Ionizable Lipid ALC-0315, a Key Component of the mRNA Vaccine Lipid Matrix

I A Boldyrev; V P Shendrikov; A G Vostrova; E L Vodovozova

doi:10.1134/S1068162023020061

A Route to Synthesize Ionizable Lipid ALC-0315, a Key Component of the mRNA Vaccine Lipid Matrix

Russ J Bioorg Chem. 2023;49(2):412-415. doi: 10.1134/S1068162023020061. Epub 2023 May 19.

Authors

I A Boldyrev¹, V P Shendrikov^{1

2}, A G Vostrova¹, E L Vodovozova¹

Affiliations

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
² Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia.

Abstract

The ionizable lipid ALC-0315, ((4-hydroxybutyl)azanediyl)bis(hexane-6,1-diyl)bis(2-hexyldecanoate), is a component of the lipid matrix of the prophylactic SARS-CoV-2 mRNA vaccine produced by Pfizer/BioNTech. This lipid ensures efficient vaccine assembly, protects the mRNA from premature degradation, and promotes the release of the nucleic acid into the cytoplasm for its further processing after endocytosis. The present work describes a simple and economical method for the synthesis of the ALC-0315 lipid, which can be taken advantage of in mRNA vaccine production.

Keywords: ALC-0315; ionizable lipid; lipid nanoparticles; mRNA; synthesis.

© The Author(s) 2023, ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2023, Vol. 49, No. 2, pp. 412–415. © The Author(s), 2023. This article is an open access publication.Russian Text © The Author(s), 2023, published in Bioorganicheskaya Khimiya, 2023, Vol. 49, No. 2, pp. 216–220.