Endophytic Colletotrichum (Sordariomycetes, Glomerellaceae) species associated with Citrusgrandis cv. "Tomentosa" in China

Jia-Wei Liu; Ishara S Manawasinghe; Xuan-Ni Liao; Jin Mao; Zhang-Yong Dong; Ruvishika S Jayawardena; Dhanushka N Wanasinghe; Yong-Xin Shu; Mei Luo

doi:10.3897/mycokeys.95.87121

Endophytic Colletotrichum (Sordariomycetes, Glomerellaceae) species associated with Citrusgrandis cv. "Tomentosa" in China

MycoKeys. 2023 Feb 23:95:163-188. doi: 10.3897/mycokeys.95.87121. eCollection 2023.

Authors

Jia-Wei Liu¹, Ishara S Manawasinghe¹, Xuan-Ni Liao¹, Jin Mao¹, Zhang-Yong Dong¹, Ruvishika S Jayawardena², Dhanushka N Wanasinghe³, Yong-Xin Shu^{1

2}, Mei Luo¹

Affiliations

¹ Innovative Institute for Plant Health/ Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
² Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand.
³ School of Science, Mae Fah Luang University, Chiang Rai, Thailand.

Abstract

Colletotrichum species are well-known plant pathogens, saprobes, endophytes, human pathogens and entomopathogens. However, little is known about Colletotrichum as endophytes of plants and cultivars including Citrusgrandis cv. "Tomentosa". In the present study, 12 endophytic Colletotrichum isolates were obtained from this host in Huazhou, Guangdong Province (China) in 2019. Based on morphology and combined multigene phylogeny [nuclear ribosomal internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (gapdh), chitin synthase 1 (chs-1), histone H3 (his3) actin (act), beta-tubulin (β-tubulin) and glutamine synthetase (gs)], six Colletotrichum species were identified, including two new species, namely Colletotrichumguangdongense and C.tomentosae. Colletotrichumasianum, C.plurivorum, C.siamense and C.tainanense are identified as being the first reports on C.grandis cv. "Tomentosa" worldwide. This study is the first comprehensive study on endophytic Colletotrichum species on C.grandis cv. "Tomentosa" in China.

Keywords: Chinese traditional medicinal plants; new ascomycete; phylogeny; six new host records; taxonomy; two new species.

Jia-Wei Liu, Ishara S. Manawasinghe, Xuan-Ni Liao, Jin Mao, Zhang-Yong Dong, Ruvishika S. Jayawardena, Dhanushka N. Wanasinghe, Yong-Xin Shu, Mei Luo.