An efficient Pt@MXene platform for the analysis of small-molecule natural products

Guanhua Zhang; Chunxia Ma; Qing He; Hongjing Dong; Li Cui; Lili Li; Lingyu Li; Yan Wang; Xiao Wang

doi:10.1016/j.isci.2023.106622

An efficient Pt@MXene platform for the analysis of small-molecule natural products

iScience. 2023 Apr 8;26(5):106622. doi: 10.1016/j.isci.2023.106622. eCollection 2023 May 19.

Authors

Guanhua Zhang^{1

2}, Chunxia Ma², Qing He³, Hongjing Dong², Li Cui², Lili Li², Lingyu Li⁴, Yan Wang¹, Xiao Wang^{1

2}

Affiliations

¹ College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, Yunnan 650500, China.
² Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China.
³ Collaborative Innovation Center of Steel Technology, University of Science and Technology Beijing, Beijing 100083, China.
⁴ Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, China.

Abstract

Small-molecule (m/z<500) natural products have rich biological activity and significant application value thus need to be effectively detected. Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) has become a powerful detection tool for small-molecule analysis. However, more efficient substrates need to be developed to improve the efficiency of SALDI MS. Thus, platinum nanoparticle-decorated Ti₃C₂ MXene (Pt@MXene) was synthesized in this study as an ideal substrate for SALDI MS in positive ion mode and exhibited excellent performance for the high-throughput detection of small molecules. Compared with using MXene, GO, and CHCA matrix, a stronger signal peak intensity and wider molecular coverage was obtained using Pt@MXene in the detection of small-molecule natural products, with a lower background, excellent salt and protein tolerance, good repeatability, and high detection sensitivity. The Pt@MXene substrate was also successfully used to quantify target molecules in medicinal plants. The proposed method has potentially wide application.

Keywords: Biomaterials; Materials property; Nanomaterials.