Unraveling Plant-Pathogen Interactions in Cereals Using RNA-seq

Methods Mol Biol. 2023:2659:103-118. doi: 10.1007/978-1-0716-3159-1_9.

Abstract

Over the past two decades, there have been significant advancements in the realm of transcriptomics, or the study of genes and their expression. Modern RNA sequencing technologies and high-performance computing are creating a "big data" revolution that provides new opportunities to explore the interactions between cereals and pathogens that affect grain yield and food safety. These data are being used to annotate genes and gene variants, as well as identify differentially expressed genes and create global gene co-expression networks. Moreover, these data can unravel the complex interactions between pathogen and host and identify genes and pathways involved in these interactions. This information can then be used for disease mitigation and the development of crops with superior resistance.

Keywords: Bioinformatics; Cereals; Fusarium graminearum; Pathogens; RNA-seq; Transcriptome; Wheat.

MeSH terms

  • Edible Grain* / genetics
  • Fusarium* / genetics
  • Host-Pathogen Interactions / genetics
  • Plant Diseases / genetics
  • RNA-Seq
  • Triticum / genetics